Analysis of secretory metabolome and transcriptome changes in radiation-treated cancer-associated fibroblasts.

Chao Bian, Lequn Wang, Zhijun Li, Ming Jia, Jie Yun, Zhijun Liu

Abstract read

In one paragraph

Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Chao BianDepartment of Infectious Diseases, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, 710061, Shaanxi Province, China.

Lequn WangSchool of life sciences, Inner Mongolia University, Hohhot, 010020, Inner Mongolia Autonomous Region, China.

Zhijun LiTumor Radiotherapy Department, Inner Mongolia People's Hospital, Hohhot, 010017, Inner Mongolia Autonomous Region, China.

Ming JiaTumor Radiotherapy Department, Inner Mongolia People's Hospital, Hohhot, 010017, Inner Mongolia Autonomous Region, China.

Jie YunTumor Radiotherapy Department, Inner Mongolia People's Hospital, Hohhot, 010017, Inner Mongolia Autonomous Region, China.

Zhijun LiuDepartment of Infectious Diseases, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, 710061, Shaanxi Province, China. zhijun_liu@xjtufh.edu.cn.

Funding

Hohhot Health Technology Program Project of China 2024-huweike-012

6 · The paper itself

Abstract

Cancer-associated fibroblasts (CAFs) constitute a predominant cellular component of the tumor microenvironment. Radiotherapy (RT) induces tumor cell death and modulates CAF biology, with irradiated CAFs potentially influencing tumor cell malignant behavior. In this study, primary CAFs were irradiated, and their supernatant was applied to A549 lung cancer cells to assess proliferation and migration. Supernatant metabolomics and CAF transcriptomics were performed to uncover paracrine mechanisms. Results showed that supernatant from irradiated CAFs significantly enhanced A549 cell proliferation and migration. Metabolomics identified 53 differentially expressed metabolites (DEMs), including glycocholic acid, which exhibited dose-dependent biphasic effects on cell viability. Pathway enrichment analysis revealed 15 (positive-mode) and 9 (negative-mode) enriched pathways. Transcriptomics identified 1,485 upregulated and 1,662 downregulated differentially expressed genes (DEGs) in irradiated CAFs. Upregulated DEGs were enriched in p53, Hippo and Wnt pathways, while downregulated DEGs were enriched in extracellular matrix-receptor interaction, focal adhesion and cell adhesion molecules. Collectively, RT reprograms the CAF secretome and transcriptome, enhancing pro-tumorigenic paracrine activity toward lung cancer cells. Limitations include lack of in vivo validation, and future work will focus on functional validation in preclinical models.

Indexed as

Cancer-Associated FibroblastsLung NeoplasmsMetabolomeTranscriptomeA549 CellsCell MovementCell ProliferationCell SurvivalGene Expression ProfilingGene Expression Regulation, NeoplasticHumansMetabolic ReprogrammingMetabolomicsTumor MicroenvironmentCancer-associated fibroblastsGlycocholic acidIrradiationMetabolomeTranscriptome

Identifiers

PMID42045472

DOI10.1038/s41598-026-50288-6

PMCPMC13287584

What Socratic holds

Textmetadata

LicenceCC BY-NC-ND

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.