ArticleBiological trace element research2026
Asparagus Saponin Officinalisnin-II Alleviates Fluoride-Induced Neurotoxicity Via Activation of the SIRT1/BDNF Signaling Pathway.
Article in Biological trace element research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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11 authors.
Funding
Abstract
Excessive fluoride exposure induces oxidative stress, inflammation, and neuronal damage, leading to cognitive dysfunction. This study investigated the neuroprotective mechanism of asparagus saponin Officinalisnin-II against fluoride-induced brain injury through modulation of the Silent Information Regulator 1 (SIRT1) / Brain Derived Neurotrophic Factor (BDNF) signaling pathway. Cytotoxicity and protective effects were evaluated in BV2 microglial cells treated with sodium fluoride (NaF) and various concentrations of Officinalisnin-II using cell viability, crystal violet, and EdU assays. Flow cytometry and Western blotting were employed to assess cell cycle, apoptosis, oxidative stress, and inflammatory markers. Expression of SIRT1/BDNF-related proteins was analyzed by Western blotting and immunofluorescence. The neurobehavioral effects were further examined in zebrafish using a T-maze test and quantitative real-time PCR (qRT-PCR). Officinalisnin-II markedly restored the proliferative activity of NaF-exposed BV2 cells, reduced apoptosis, and normalized cell-cycle progression. It significantly decreased intracellular reactive oxygen species and malondialdehyde levels, enhanced superoxide dismutase, glutathione peroxidase, and total antioxidant capacity, and suppressed interleukin-1 and interleukin-6 expression. The treatment upregulated SIRT1, BDNF, tropomyosin receptor kinase B (TrkB), phosphatidylinositol 3-kinase (PI3K), protein kinase B (AKT), while downregulating forkhead box protein O1A (FOXO1A), indicating activation of the SIRT1/BDNF pathway. In zebrafish, Officinalisnin-II improved learning and memory performance and increased expression of neuroprotective genes. These findings demonstrate that asparagus saponin Officinalisnin-II effectively mitigates fluoride-induced neurotoxicity by enhancing antioxidant capacity, reducing inflammation, and activating the SIRT1/BDNF signaling cascade, providing experimental evidence for its potential use in preventing fluorosis-related brain injury.
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Registered trials
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