ArticlePloS one2026
Comparative genomics-based development of a LAMP assay for rapid and reliable in-field detection of Fusarium oxysporum f. sp. cubense tropical race 4.
Article in PloS one, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- Genetic and Pathogenic Differentiation ofJournal of fungi (Basel, Switzerland) · 2026Article
- Robust design of LAMP assays for in-field detection of major bacterial vascular diseases of banana.PloS one · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
22 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fusarium wilt, caused by the soilborne fungus Fusarium oxysporum f. sp. cubense (Foc) Tropical race 4 (TR4) is currently devastating Cavendish banana production worldwide. The availability of accurate and rapid field-deployable detection tools is vital for effective disease prevention and containment practices, as no effective treatment is currently available. Design of reliable molecular detection tools, however, is challenged by genetic similarity between non-pathogenic and target Foc strains that are present in symptomatic plant material. The high genetic diversity present among Foc isolates further complicates the identification of unique genomic regions suitable for diagnosis. Current molecular assays are also still mostly confined to laboratory settings and rely on expensive equipment that needs skilled operators to perform. In the present study, a simple loop-mediated isothermal amplification (LAMP) detection assay was developed for direct in-field detection. A comprehensive database of Foc genomes including 148 TR4 genomes and 146 non-target genomes was used, considering the wide diversity within Foc. These genomic sequences were exploited in a comparative genomics in silico pipeline using the KEC method (K-mer elimination by cross-reference), combined with filtering steps using BLASTn, and Illumina read alignments. The specificity of the LAMP primers was validated using a diverse collection of 161 isolates, which included Foc-TR4 isolates, Foc VCGs, F. oxysporum spp. endophytes, other Fusarium spp., and genera which are commonly isolated from banana vascular tissue. The limit of detection reached 102 copies µL-1 of plasmid DNA, and the target pathogen was successfully identified both from artificially inoculated and naturally infested material in contrasted environments, indicating the LAMP assay to be a robust and reliable tool fully suitable for field diagnosis.
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.