Evidence map›Paper›PMID 42055327›Full record

ArticleThe Journal of biological chemistry2026

Genome-wide CRISPR interference screen identifies Clip2 as a novel regulator of osteocyte maturation and morphology.

Courtney M Mazur, Parthena E Kotsalidis, Majd George, Tom Whalley, Tadatoshi Sato, John G Doench, Lauren E Surface, Marc N Wein

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of biological chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors.

Courtney M MazurEndocrine Unit, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA.
Parthena E KotsalidisEndocrine Unit, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA.
Majd GeorgeEndocrine Unit, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA.
Tom WhalleyEndocrine Unit, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA; Wellcome Centre for Cell-Matrix Research, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, UK.
Tadatoshi SatoEndocrine Unit, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA; Division of Rheumatology, Department of Medicine, University of Massachusetts Chan Medical School, Worcester, Massachusetts, USA.
John G DoenchBroad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.
Lauren E SurfaceEndocrine Unit, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA; Department of Biologic and Materials Sciences & Prosthodontics, University of Michigan School of Dentistry, Ann Arbor, Michigan, USA.
Marc N WeinEndocrine Unit, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA; Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA. Electronic address: mnwein@mgh.harvard.edu.

Funding

TRAINING PROGRAM IN ENDOCRINOLOGY AND DIABETEST32DK007028 · NIDDK · MASSACHUSETTS GENERAL HOSPITAL · PI Karen K Miller · 1986 to 2026
$18.5M
The role of salt inducible kinases in parathyroid hormone action in boneR01DK116716 · NIDDK · MASSACHUSETTS GENERAL HOSPITAL · PI Marc Nathan Wein · 2018 to 2026
$3.8M
Developing and evaluating bone targeting agents to mimic the skeletal effects of mechanical loadingR21AR079633 · NIAMS · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI SATO, TADATOSHI · 2022 to 2023
$457k
Precision Gene Editing on Osteogenesis ImperfectaR21AR084644 · NIAMS · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI SATO, TADATOSHI, SHIM, JAE-HYUCK · 2024 to 2025
$387k
Trafficking and translation of mRNA in osteocyte dendritesF32AR081660 · NIAMS · MASSACHUSETTS GENERAL HOSPITAL · PI MAZUR, COURTNEY MARISA · 2022 to 2024
$91k
NIAMS NIH HHS F32 AR081660NIAMS NIH HHS R21 AR079633NIAMS NIH HHS R21 AR084644NIDDK NIH HHS R01 DK116716NIDDK NIH HHS T32 DK007028
6 · The paper itself

Abstract

Osteocytes play critical roles in bone, making them attractive targets for therapeutics aimed at improving bone mass and strength. The genes driving osteocyte maturation and function are not fully understood. Here, we aimed to identify novel genes responsible for osteocyte differentiation and dendrite development by performing a genome-wide CRISPR-interference (CRISPRi) screen in the Ocy454 osteocyte-like cell line. We identify CD61 (integrin β3) as a marker of osteocyte maturation: surface CD61 expression increases during osteocyte maturation, and CD61

Indexed as

Clustered Regularly Interspaced Short Palindromic RepeatsCRISPR-Cas SystemsMicrotubule-Associated ProteinsOsteocytesAdaptor Proteins, Signal TransducingAnimalsCell DifferentiationCell LineExtracellular Matrix ProteinsMiceAdaptor Proteins, Signal TransducingDmp1 protein, mouseExtracellular Matrix ProteinsMicrotubule-Associated ProteinsSost protein, mousecytoskeletongenome-wide CRISPR screenosteocyte

Identifiers

PMID42055327
PMCPMC13254583

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.