Evidence map›Paper›PMID 42056969›Full record

ArticleBMC complementary medicine and therapies2026

Phytochemical investigation and evaluation of anti-inflammatory and wound healing activities of Plantago major subsp. intermedia (Gilib.) Lange.

Hilal Bacanak, Zeynep Dogan, Esra Küpeli, Akito Nagatsu, Iclal Saracoglu

Abstract read
In one paragraph

Article in BMC complementary medicine and therapies, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Hilal BacanakFaculty of Pharmacy, Department of Pharmacognosy, Hacettepe University, Ankara, 06100, Türkiye. hilal.bacanak@hacettepe.edu.tr.ORCID http://orcid.org/0000-0002-8571-8358
Zeynep DoganFaculty of Pharmacy, Department of Pharmacognosy, Hacettepe University, Ankara, 06100, Türkiye.ORCID http://orcid.org/0000-0002-0108-6040
Esra KüpeliFaculty of Pharmacy, Department of Pharmacognosy, Gazi University, Ankara, 06330, Türkiye.ORCID http://orcid.org/0000-0002-5829-7869
Akito NagatsuCollege of Pharmacy, Department of Pharmacognosy, Kinjo Gakuin University, Nagoya, 463‑8521, Japan.ORCID http://orcid.org/0000-0003-3863-0991
Iclal SaracogluFaculty of Pharmacy, Department of Pharmacognosy, Hacettepe University, Ankara, 06100, Türkiye.ORCID http://orcid.org/0000-0003-0555-6262

Funding

Hacettepe University BAP THD-2021-19519Türkiye Bilimsel ve Teknolojik Araştırma Kurumu 1059B142300168
6 · The paper itself

Abstract

backgroundPlantago major subsp. intermedia has been traditionally used in Türkiye for the treatment of wounds, abscesses, constipation, pain, ulcers, hemorrhoids and gynecological diseases. In the present study, the phytochemical composition of P. major subsp. intermedia (Gilib.) Lange was investigated and its anti-inflammatory and wound healing effects were examined in light of existing experimental evidence from related Plantago species.

methodsMeOH, 80% EtOH and water extracts of the plant were prepared. In vivo anti-inflammatory effects of these extracts were investigated using an acetic acid-induced capillary permeability model and their wound healing effects were studied using linear incision and circular excision wound models. Isolation studies were performed on the 80% EtOH extract. Furthermore, in vitro studies were conducted on the 80% EtOH extract, the main fractions obtained from this extract and the some of the isolated pure compounds. In these in vitro studies, anti-inflammatory effects were investigated by measuring nitric oxide (NO), interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) levels and wound healing effects were investigated using the scratch test.

resultsA total of six compounds were isolated from P. major subsp. intermedia: Isotachioside (1), aucubin (2), 10-hydroxymajoroside (3), 10-acetoxymajoroside (4), martynoside (5) and acteoside (6). All tested compounds significantly inhibited NO production at concentrations of 25, 50 and 100 µM (12.68%-61.89%). Although in vivo results were not statistically significant, the 80% EtOH extract showed the highest efficacy in linear incision and circular excision wound models.

conclusionsThe results of this study indicate the in vitro anti-inflammatory and wound healing activities of P. major subsp. intermedia and are consistent with previously reported biological activities of P. major.

Indexed as

Anti-Inflammatory AgentsPhytochemicalsPlantagoPlant ExtractsWound HealingAnimalsHumansMaleMiceRatsAnti-Inflammatory AgentsPhytochemicalsPlant ExtractsAnti-inflammatory ActivityPhytochemistryPlantago major subsp. intermediaWound Healing Activity

Identifiers

PMID42056969
PMCPMC13277164

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.