Evidence mapPaperPMID 42057340Full record

ArticleBioMed research international2026

The Effects of Neonatal Zingerone Administration and Adolescent Alcohol Exposure on Bone Health Markers and Morphometry in Male Sprague-Dawley Rats.

Brunhildé De Vos, Bernice Asiedu, Anna M Joubert, Abe E Kasonga, Trevor T Nyakudya

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In one paragraph

Article in BioMed research international, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Brunhildé De VosDepartment of Human Physiology, School of Medicine, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa, up.ac.za.ORCID https://orcid.org/0000-0003-3122-9812
Bernice AsieduDepartment of Human Physiology, School of Medicine, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa, up.ac.za.ORCID https://orcid.org/0000-0001-6407-7566
Anna M JoubertDepartment of Human Physiology, School of Medicine, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa, up.ac.za.ORCID https://orcid.org/0000-0002-6931-7554
Abe E KasongaDepartment of Human Physiology, School of Medicine, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa, up.ac.za.ORCID https://orcid.org/0000-0001-7379-6044
Trevor T NyakudyaDepartment of Human Physiology, School of Medicine, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa, up.ac.za.ORCID https://orcid.org/0000-0003-1872-9257

Funding

National Research Foundation 121828
6 · The paper itself

Abstract

objectiveNeonatal interventions influence long-term developmental outcomes, with excessive alcohol consumption impairing metabolism and bone health. Zingerone, a natural antioxidant, may possess health benefits against alcohol-induced oxidative damage and inflammation, promoting bone health. This study investigates the effects of zingerone on markers of bone turnover and morphometry in neonatal rats subjected to alcohol exposure during critical developmental stages.

methodsTen-day-old male Sprague-Dawley rats (N = 35) were randomized and treated with water (C), zingerone (Z) (40 mg/kg), alcohol (A) (1 g/kg) or zingerone-alcohol combination (ZA) for 9 days. During adolescence, rats received either water or a secondary A insult (20% v/v) for 54 days. At termination, ELISA kits were used to measure biomarkers of bone formation (bone-specific alkaline phosphatase, osteocalcin, and procollagen Type I N-terminal propeptide). Bone morphology and morphometry was assessed using microcomputed tomography.

resultsNeonatal zingerone (Z + A; ZA + A) and double alcohol exposure (A + A) significantly increased plasma BALP and P1NP levels (p < 0.05). Tibial length remained unchanged across groups (p > 0.05). Neonatal zingerone with adolescent alcohol (Z + A) significantly reduced tibial mass, bone mass-to-length ratios, and trabecular architecture near the growth plate (p < 0.05). Neonatal and adolescent administration of alcohol (A + A) significantly increased midshaft cortical thickness (p < 0.05).

conclusionAdolescent alcohol treatment impaired bone function and morphology, neonatal zingerone alone offering limited protection. Coadministration of zingerone with alcohol neonatally produced variable and context-dependent changes with no clear evidence of sustained protection against the effects of alcohol exposure. These findings underscore the importance of optimizing timing, dosage, and combination therapies for bone health. Further studies are warranted to clarify whether zingerone has clinically meaningful bone-protective effects.

Indexed as

Bone and BonesEthanolGuaiacolAlkaline PhosphataseAnimalsAnimals, NewbornBiomarkersBone DensityMaleOsteocalcinOsteogenesisRatsRats, Sprague-DawleyX-Ray MicrotomographyAlkaline PhosphataseBiomarkersEthanolGuaiacolOsteocalcinzingeronealcohol consumptionbone healthbone microarchitecturezingerone

Identifiers

PMID42057340
PMCPMC13128980

What Socratic holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.