Evidence map›Paper›PMID 42062895›Full record

ArticleBMC genomics2026

Identification of TSHB gene expression profile in Duolang sheep and its functional role in granulosa cells.

HuiPing Sun, LeXiao Zhu, GulMuhammad Shahbaz, RuoHuai Gu, ChengLong He, ShuXin Chen, ChaoFan Wang, Feng Xing, XiangLin Yan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC genomics, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

HuiPing SunKey Laboratory of Tarim Animal Husbandry Science and Technology, Xinjiang Production and Construction Group, School of Animal Science and Technology, Tarim University, Alaer, 843300, China.ORCID http://orcid.org/0009-0004-5545-6344
LeXiao ZhuKey Laboratory of Tarim Animal Husbandry Science and Technology, Xinjiang Production and Construction Group, School of Animal Science and Technology, Tarim University, Alaer, 843300, China.ORCID http://orcid.org/0009-0009-6400-7483
GulMuhammad ShahbazKey Laboratory of Tarim Animal Husbandry Science and Technology, Xinjiang Production and Construction Group, School of Animal Science and Technology, Tarim University, Alaer, 843300, China.ORCID http://orcid.org/0009-0008-0886-8863
RuoHuai GuKey Laboratory of Tarim Animal Husbandry Science and Technology, Xinjiang Production and Construction Group, School of Animal Science and Technology, Tarim University, Alaer, 843300, China.ORCID http://orcid.org/0009-0006-4447-3178
ChengLong HeKey Laboratory of Tarim Animal Husbandry Science and Technology, Xinjiang Production and Construction Group, School of Animal Science and Technology, Tarim University, Alaer, 843300, China.ORCID http://orcid.org/0009-0009-8523-4386
ShuXin ChenKey Laboratory of Tarim Animal Husbandry Science and Technology, Xinjiang Production and Construction Group, School of Animal Science and Technology, Tarim University, Alaer, 843300, China.ORCID http://orcid.org/0009-0008-6595-045X
ChaoFan WangKey Laboratory of Tarim Animal Husbandry Science and Technology, Xinjiang Production and Construction Group, School of Animal Science and Technology, Tarim University, Alaer, 843300, China.ORCID http://orcid.org/0009-0008-3438-0781
Feng XingKey Laboratory of Tarim Animal Husbandry Science and Technology, Xinjiang Production and Construction Group, School of Animal Science and Technology, Tarim University, Alaer, 843300, China. xingfeng2001@126.com.ORCID http://orcid.org/0000-0001-5209-9143
XiangLin YanKey Laboratory of Tarim Animal Husbandry Science and Technology, Xinjiang Production and Construction Group, School of Animal Science and Technology, Tarim University, Alaer, 843300, China. yanxianglin12@163.com.ORCID http://orcid.org/0009-0005-2459-096X

Funding

Major Science and Technology Special Project of Xinjiang Uygur Autonomous Region 2024A02004-3National Natural Science Foundation of China 31960655Postgraduate Practical Innovation Project of Xinjiang Production and Construction Corps BTYJXM-2024-S23resident's Fund Project of Tarim University TDZKCX202401
6 · The paper itself

Abstract

objectiveThyroid-stimulating hormone beta subunit (TSHB) gene, a member of the glycoprotein hormone beta subunit family, has been detected in ovarian tissues and granulosa cells (GCs) of several species, suggesting a potential involvement in ovarian function. However, its molecular regulatory mechanisms and functional roles in Duolang sheep remain unclear.

methodsClone the coding sequence of the TSHB gene in Duolang sheep and identify the development stage when its expression is high. Models with TSHB overexpression and siRNA interference were constructed to investigate their effects on the proliferation, apoptosis, and hormone secretion during the follicular phase in ovarian GCs in Duolang sheep.

resultsCCK-8 assays, conducted at four distinct time points, showed that TSHB overexpression significantly enhanced the proliferation of GCs, accompanied by the upregulation of Cyclin E/CDK2 and Bcl-2, and downregulation of Bax and Caspase3. Interference showed a trend opposite to that of overexpression (p < 0.05). ELISA results revealed that TSHB overexpression significantly reduced estrogen levels. Interference showed a trend opposite to that of overexpression (p < 0.05), which was consistent with decreased expression of steroidogenic acute regulatory protein and CYP19A1. Additionally, qPCR analysis demonstrated that TSHB significantly upregulated the expression of TSHR and FSHR (p < 0.05).

conclusionSpecifically, the above results indicate that the TSHB gene promotes the proliferation of GCs in Duolang sheep, providing a new perspective on their follicular development.

Indexed as

Gene Expression ProfilingGranulosa CellsThyrotropin, beta SubunitAnimalsApoptosisCell ProliferationFemaleGene Expression RegulationSheepThyrotropin, beta SubunitDuolang sheepGCsTSHB gene

Identifiers

PMID42062895
PMCPMC13134140

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.