Evidence map›Paper›PMID 42071207›Full record

ArticleVirology journal2026

ZDHHC11-mediated palmitoylation of viral envelope protein restricts dengue virus replication: implication for the development of universal anti-flavivirus therapeutic strategies.

Mingshuang Lai, Lexin Zhang, Rongji Lai, Qin Du, Shilin Li, Jinsheng Liu, Baoren He, Bin Li, Limin Chen

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Virology journal, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Mingshuang Lai *Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College, Chengdu, China.
Lexin Zhang *Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College, Chengdu, China.
Rongji LaiThe Joint Laboratory on Transfusion-transmitted Diseases (TTDs) Between Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Nanning Blood Center, Nanning Blood Center, Nanning, China.
Qin DuDepartment of Clinical Medicine, North Sichuan Medical College, Nanchong, Sichuan, China.
Shilin LiInstitute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College, Chengdu, China.
Jinsheng LiuInstitute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College, Chengdu, China.
Baoren HeThe Joint Laboratory on Transfusion-transmitted Diseases (TTDs) Between Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Nanning Blood Center, Nanning Blood Center, Nanning, China.
Bin LiThe Joint Laboratory on Transfusion-transmitted Diseases (TTDs) Between Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Nanning Blood Center, Nanning Blood Center, Nanning, China.
Limin ChenInstitute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College, Chengdu, China. limin_chen_99@126.com.ORCID http://orcid.org/0000-0001-5228-3522

Funding

the Fundamental Research Funds for Central Universities, Peking Union Medical College 3332025144the Sichuan Science and Technology Program 2025ZNSFSC0678the Youth Science Foundation of the Guangxi Natural Science Foundation 2024GXNSFBA010047
6 · The paper itself

Abstract

Palmitoylation is a key post-translational modification regulating viral replication, yet its regulatory mechanism in Dengue virus (DENV) infection remains elusive. This study aimed to elucidate the underlying regulatory function of palmitoylation and zinc finger Asp-His-His-Cys (ZDHHC) proteins in DENV replication and identify palmitoylated DENV proteins. We explored the function of palmitoylation in DENV replication using the palmitoylation inhibitor 2-bromopalmitate (2BP) and enhancer palmitic acid (PA), combined with qRT-PCR, western blot, confocal immunofluorescence microscopy, co-immunoprecipitation, and acyl-biotin exchange (ABE) assays. We found that 2BP promoted DENV replication, while PA inhibited it. Further analyses showed that 2BP and PA exerted no significant effects on DENV adsorption, internalization, or the interactions between E protein and the structural proteins prM/C that are essential for viral assembly. ABE assays verified that the DENV E protein is palmitoylated, with no such modification detected in prM and C proteins. Mass spectrometry and site-directed mutagenesis further revealed that the DENV E protein is palmitoylated at three cysteine residues: Cys74, Cys302, and Cys333. ZDHHC11, a characterized regulator of Zika virus E protein palmitoylation, directly interacted with DENV E protein and catalyzed its palmitoylation. Functionally, knockdown of ZDHHC11 enhanced DENV replication, while overexpression suppressed it. These findings deepen our understanding of flavivirus-host interaction mechanisms and provide a theoretical basis for the development of broad-spectrum anti-flavivirus therapies.

Indexed as

AcyltransferasesDengue VirusLipoylationViral Envelope ProteinsVirus ReplicationAnimalsCell LineDengueHumansPalmitatesPalmitic AcidProtein Processing, Post-Translational2-bromopalmitateAcyltransferasesPalmitatesPalmitic AcidViral Envelope ProteinsDengue virusEnvelope proteinPalmitoylationPalmitoyltransferaseViral replication

Identifiers

PMID42071207
PMCPMC13288571

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.