Evidence map›Paper›PMID 42071316›Full record

ArticleNano letters2026

Reconstruction of Molecular Interaction Patterns from Endolysosomes in Ceramide-Depleted Cells.

Yiqing Feng, Florian Gärber, Cecilia Spedalieri, Stephan Werner, Christoph Pratsch, Christoph Arenz, Stephan Seifert, Janina Kneipp

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nano letters, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Yiqing FengDepartment of Chemistry, Humboldt-Universität zu Berlin, Brook-Taylor-Str. 2, 12489 Berlin, Germany.
Florian GärberHamburg School of Food Science, Department of Chemistry, Universität Hamburg, Grindelallee 117, 20146 Hamburg, Germany.
Cecilia SpedalieriDepartment of Chemistry, Humboldt-Universität zu Berlin, Brook-Taylor-Str. 2, 12489 Berlin, Germany.ORCID 0000-0001-7480-0404
Stephan WernerHelmholtz-Zentrum Berlin für Materialien und Energie GmbH, Department X-ray Microscopy, Albert-Einstein-Str. 15, 12489 Berlin, Germany.
Christoph PratschHelmholtz-Zentrum Berlin für Materialien und Energie GmbH, Department X-ray Microscopy, Albert-Einstein-Str. 15, 12489 Berlin, Germany.ORCID 0000-0003-4315-4854
Christoph ArenzDepartment of Chemistry, Humboldt-Universität zu Berlin, Brook-Taylor-Str. 2, 12489 Berlin, Germany.
Stephan SeifertHamburg School of Food Science, Department of Chemistry, Universität Hamburg, Grindelallee 117, 20146 Hamburg, Germany.
Janina KneippDepartment of Chemistry, Humboldt-Universität zu Berlin, Brook-Taylor-Str. 2, 12489 Berlin, Germany.ORCID 0000-0001-8542-6331

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Ceramide synthase (CerS) is a key enzyme in sphingolipid metabolism, predominantly maintaining lipid homeostasis within the endoplasmic reticulum (ER). Capturing the biochemical consequences of CerS activity at the subcellular level is of great interest to understand complex lipid flux in living cells. Here, surface-enhanced Raman scattering (SERS) combined with soft X-ray tomography and random forest (RF) machine learning was employed to probe the cellular response to CerS inhibition. The nanospectroscopy data from endolysosomes and changes in ultrastructure demonstrate diverging alterations upon the sequential administration of CerS inhibitor Fumonisin B1 and SERS probe gold nanoparticles. RF analysis reveals distinct spectral patterns of the affected endolysosomes, characteristic of a ceramide imbalance under different conditions, indicating a tight metabolic coupling between the ER and endolysosomes. The vibrational nanospectroscopy coupled with computational reconstruction of molecular interaction patterns and ultrastructural imaging provides a comprehensive strategy to understand enzyme activity and metabolic dysregulation in situ.

Indexed as

CeramidesEndosomesOxidoreductasesEndoplasmic ReticulumFumonisinsGoldHumansMetal NanoparticlesRandom ForestSpectrum Analysis, RamanCeramidesdihydroceramide desaturasefumonisin B1FumonisinsGoldOxidoreductasesceramideceramide synthase (CerS)Fumonisin B1gold nanoparticlessurface enhanced Raman scattering (SERS)surrogate minimal depth (SMD)

Identifiers

PMID42071316
PMCPMC13178127

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.