ArticleInternational journal of molecular sciences2026
FTIR Spectroscopy of Vitreous Humor for Postmortem Interval Estimation: A Multivariate Regression Approach.
Article in International journal of molecular sciences, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Estimation of the postmortem interval (PMI) remains a major challenge in forensic science. We used attenuated total reflection (ATR)-Fourier-transform infrared (FTIR) spectroscopy combined with chemometric modeling for PMI prediction using vitreous humor samples from 20 forensic cases with known PMI (24.8-97.6 h) and 10 with unknown PMI. The intensities of vibrational bands commonly associated with PMI were analyzed, and several peaks in the carbohydrate/phosphate region showed significant correlations with PMI. Principal component analysis revealed time-dependent spectral evolution, with PC1 (48.1%) associated mainly with carbohydrate/phosphate variations and PC2 (37.6%) with protein structural changes. Partial least squares regression with two latent variables achieved a cross-validated RMSE of 15.8 h (R
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