Holo-2bRAD: A Hologenomic Method for High-Resolution Analysis of Coral Microbiomes During Bleaching.

Zhuqing Wang, Cen Ma, Heng Huang, Shaowen Ke, Jia Lv, Jingjie Hu, Shi Wang, Zhenmin Bao

Abstract read

In one paragraph

Article in Microorganisms, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

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2 · The registry

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0 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Zhuqing WangSouthern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou 511458, China.

Cen MaFang Zongxi Center for Marine Evo-Devo, MOE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266100, China.ORCID 0009-0004-8751-3120

Heng HuangKey Laboratory of Tropical Aquatic Germplasm of Hainan Province, Sanya Oceanographic Institution, Ocean University of China, Sanya 572024, China.

Shaowen KeSanya Coral Reef Ecology Institute, Sanya 572025, China.

Jia LvFang Zongxi Center for Marine Evo-Devo, MOE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266100, China.

Jingjie HuSouthern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou 511458, China.

Shi WangSouthern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou 511458, China.

Zhenmin BaoSouthern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou 511458, China.

Funding

Department of Science and Technology of Hainan Province SOLZSKY2025013GCI Science & Technology (China) 2025A04J3824Guangdong S&T Program 2025B1111180001

6 · The paper itself

Abstract

Coral reefs are biodiversity hotspots increasingly threatened by climate-induced bleaching, yet profiling the coral holobiont-the host and its associated microbiota-remains technically challenging due to high host-DNA contamination (often >95%) and the lack of comprehensive reference databases. Here, we present holo-2bRAD, a type IIB restriction site-associated DNA sequencing approach. This method, strategically integrated with a meticulously curated hologenome database (comprising 404,946 microbial genomes and 56 coral-derived metagenome-assembled genomes), effectively overcomes overwhelming host contamination (~99%). We demonstrate its exceptional species specificity (99.92%) in profiling

Indexed as

coral bleachingholo-2bRADhologenomemicrobial community

Identifiers

PMID42075236

DOI10.3390/microorganisms14040840

PMCPMC13118862

What Socratic holds

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LicenceCC BY

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.