ArticleScientific data2026
Global dataset of soil eukaryotic communities created with a uniform protocol and long read sequencing.
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136 authors.
Vladimir MikryukovDepartment of Botany, Institute of Ecology and Earth Sciences, University of Tartu, Juhan Liivi 2, Tartu, 50409, Estonia. vladimir.mikryukov@ut.ee.ORCID http://orcid.org/0000-0003-2786-2690
Olesya DulyaDepartment of Botany, Institute of Ecology and Earth Sciences, University of Tartu, Juhan Liivi 2, Tartu, 50409, Estonia.ORCID http://orcid.org/0000-0002-7185-8659
Kessy AbarenkovMycology and Microbiology Center, University of Tartu, Juhan Liivi 2, Tartu, 50409, Estonia.ORCID http://orcid.org/0000-0001-5526-4845
Sten AnslanMycology and Microbiology Center, University of Tartu, Juhan Liivi 2, Tartu, 50409, Estonia.ORCID http://orcid.org/0000-0002-2299-454X
Niloufar Hagh-DoustDepartment of Botany, Institute of Ecology and Earth Sciences, University of Tartu, Juhan Liivi 2, Tartu, 50409, Estonia.ORCID http://orcid.org/0000-0003-0616-5829
Victoria PrinsMycology and Microbiology Center, University of Tartu, Juhan Liivi 2, Tartu, 50409, Estonia.ORCID http://orcid.org/0009-0003-8968-6773
Kristel PanksepInstitute of Technology, Faculty of Science and Technology, University of Tartu, Nooruse 1, Tartu, 50411, Estonia.ORCID http://orcid.org/0000-0003-4743-6111
Sergei PõlmeMycology and Microbiology Center, University of Tartu, Juhan Liivi 2, Tartu, 50409, Estonia.ORCID http://orcid.org/0000-0002-9658-1166
Khalid S IbrahimDepartment of Biology, College of Science, University of Zakho, Zakho, Kurdistan Region, 42002, Iraq.ORCID http://orcid.org/0000-0002-8585-3429
Mo BahramDepartment of Agroecology, Aarhus University, Forsøgsvej 1, Slagelse, 4200, Denmark.ORCID http://orcid.org/0000-0002-9539-3307
Kalev AdamsonChair of Silviculture and Forest Ecology, Institute of Forestry and Engineering, Estonian University of Life Sciences, F. R. Kreutzwaldi 5, Tartu, 51006, Estonia.ORCID http://orcid.org/0000-0002-8810-8838
Ahto AganChair of Silviculture and Forest Ecology, Institute of Forestry and Engineering, Estonian University of Life Sciences, F. R. Kreutzwaldi 5, Tartu, 51006, Estonia.
Talaat AhmedEnvironmental Science Center, Qatar University, Doha, Qatar.ORCID http://orcid.org/0000-0001-8022-1855
Juha M AlataloEnvironmental Science Center, Qatar University, Doha, Qatar.ORCID http://orcid.org/0000-0001-5084-850X
Felipe E AlbornozSchool of Biological Sciences, The University of Western Australia, 35 Stirling Hwy, Crawley, WA, 6009, Australia.ORCID http://orcid.org/0000-0001-9526-0945
Abdullah Ms Al-HatmiNatural and Medical Sciences Research Center, University of Nizwa, Nizwa, 616, Oman.ORCID http://orcid.org/0000-0002-5206-2647
Saad AlkahtaniDepartment of Zoology, College of Science, King Saud University, P.O. Box 2455, Riyadh, 11451, Saudi Arabia.ORCID http://orcid.org/0000-0001-7381-5110
Julieta Alvarez-ManjarrezMicología Integral, Instituto de Biología, Universidad Nacional Autónoma de México, Tercer circuito interior s/n, Ciudad Universitaria, Coyoacán, Mexico City, 04510, México.ORCID http://orcid.org/0000-0002-5581-7443
Jelena AnkudaVokė Branch, Institute of Agriculture, Lithuanian Research Centre for Agriculture and Forestry, Žalioji Sq. 2, Vilnius, LT-02232, Lithuania.ORCID http://orcid.org/0000-0002-9446-7802
Alexandre AntonelliRoyal Botanic Gardens, Kew GB, Richmond, Surrey, TW9 3AE, UK.ORCID http://orcid.org/0000-0003-1842-9297
Manikandan AriyanDepartment of Botany, Institute of Ecology and Earth Sciences, University of Tartu, Juhan Liivi 2, Tartu, 50409, Estonia.ORCID http://orcid.org/0000-0002-7277-0544
Kęstutis ArmolaitisDepartment of Silviculture and Ecology, Institute of Forestry, Lithuanian Research Centre for Agriculture and Forestry, Liepų str. 1, Girionys, Kaunas distr., LT-53101, Lithuania.ORCID http://orcid.org/0000-0001-8295-2440
Farzad AslaniAbove-belowground interactions group, Institute of Biology, Leiden University, Rapenburg 70, Leiden, Netherlands.ORCID http://orcid.org/0000-0003-0485-9800
Isabel C BarrioFaculty of Life and Land, Agricultural University of Iceland, Árleyni 22, Reykjavík, Iceland, 112, Iceland.ORCID http://orcid.org/0000-0002-8120-5248
Marijn BautersQ-ForestLab, Department of Environment, Ghent University, Coupure Links 653, Gent, 9000, Belgium.ORCID http://orcid.org/0000-0003-0978-6639
Elisabeth Machteld BiersmaNatural History Museum of Denmark, Øster Voldgade 5-7, Copenhagen K, 1350, Denmark.ORCID http://orcid.org/0000-0002-9877-2177
Krišs BitenieksLatvian State Forest Research Insititute "Silava", Rīga str. 111, Salaspils, LV-2169, Latvia.ORCID http://orcid.org/0000-0001-8829-3263
Gregory BonitoDepartment of Plant Soil and Microbial Sciences, Michigan State University, Michigan, MI, 48824, USA.
Francis Q BrearleyDepartment of Natural Sciences, Manchester Metropolitan University, Chester Street, Manchester, M1 5GD, UK.ORCID http://orcid.org/0000-0001-5053-5693
Kari Anne BråthenDepartiment of Arctic and Marine Ecology, UiT The Arctic University of Norway, Breivika, Tromsø, 9037, Norway.ORCID http://orcid.org/0000-0003-0942-1074
Franz BueggerResearch Unit Environmental Simulation, Helmholtz Zentrum München, Ingolstädter Landstraße 1, Neuherberg, 85764, Germany.ORCID http://orcid.org/0000-0003-3526-4711
Klaus Butterbach-BahlAgroecology, Department, Aarhus University, Ole Worms Alle 3, Aarhus, 8000, Denmark.ORCID http://orcid.org/0000-0001-9499-6598
Miklós BálintFunctional Environmental Genomics, Senckenberg Biodiversity and Climate Research Centre, Georg-Voigt-Str. 14-16, Frankfurt am Main, Hesse, 60325, Germany.ORCID http://orcid.org/0000-0003-0499-8536
Erin K CameronDepartment of Biology, Saint Mary's University, 923 Robie St, Halifax, Nova Scotia, B3H 3C3, Canada.ORCID http://orcid.org/0000-0002-3374-9830
Fabiana CaniniDepartment of Ecological and Biological Sciences, University of Tuscia, Largo dell'Università, Viterbo, 01100, Italy.ORCID http://orcid.org/0000-0002-9626-6318
Rebeca Casique-ValdésHorticulture department, Universidad Autónoma Agraria Antonio Narro, Saltillo, Coahuila, 25315, México.ORCID http://orcid.org/0000-0002-0497-8255
Adriana CorralesSociety for the Protection of Underground Networks (SPUN), 500 South DuPont Highway, Dover, DE, 19901, USA.ORCID http://orcid.org/0000-0001-9885-4634
Evgeny A Davydovno affiliation, Barnaul, Russia.ORCID http://orcid.org/0000-0002-2316-8506
Eske De CropDepartment of Biology, Ghent University, K.L. Ledeganckstraat 35, Gent, 9000, Belgium.ORCID http://orcid.org/0000-0002-9067-6981
André De KeselResearch Department, Biodiversity and Evolution, Meise Botanic Garden, Nieuwelaan 38, Meise, B-1860, Belgium.ORCID http://orcid.org/0000-0002-5287-4637
Joseph Fovo DjeugapDepartment of Crop Sciences, Plant Protection, University of Dschang, Dschang, West Region, PO. Box 222, Cameroon.ORCID http://orcid.org/0000-0003-4786-7026
Rein DrenkhanChair of Silviculture and Forest Ecology, Institute of Forestry and Engineering, Estonian University of Life Sciences, F. R. Kreutzwaldi 5, Tartu, 51006, Estonia.
Camila Duarte RitterIntituto Juruá, Manaus, Amazonas, 69083-300, Brazil.ORCID http://orcid.org/0000-0002-3371-7425
Sergey V Dudovno affiliation, Moscow, Russia.ORCID http://orcid.org/0000-0003-1512-0956
Mikk EspenbergDepartment of Geography, Institute of Ecology and Earth Sciences, University of Tartu, Vanemuise 46, Tartu, 51003, Estonia.ORCID http://orcid.org/0000-0003-0469-6394
Ongua FanuelSoils, Environment and Agro-meteorology, National Agricultural Research Laboratories-Kawanda, National Agricultural Research Organization NARO, Kampala, 7065, Uganda.ORCID http://orcid.org/0009-0002-4902-4858
Vladimir E Fedosovno affiliation, Moscow, Russia.ORCID http://orcid.org/0000-0002-5331-6346
Luke FlorenceDepartment of Ecological, Plant and Animal Sciences, La Trobe University, Bundoora, Victoria, 3086, Australia.ORCID http://orcid.org/0000-0002-1901-7772
Brendan R FurneauxDepartment of Biological and Environmental Science, Faculty of Mathematics and Science, University of Jyväskylä, Survontie 9 C, Jyväskylä, Central Finland, 40500, Finland.ORCID http://orcid.org/0000-0003-3522-7363
Ariadne N M FurtadoDepartment of Biomedical Sciences, University of Cagliari, Cittadella Universitaria, Monserrato, CA, I09042, Italy.ORCID http://orcid.org/0000-0001-9596-7553
Sanni FärkkiläUniversity of Tartu, Ülikooli 18a, Tartu, 51005, Estonia.ORCID http://orcid.org/0000-0002-4485-5567
Natalia S Gamovano affiliation, Moscow, Russia.ORCID http://orcid.org/0000-0002-4141-757X
Roberto Garibay-OrijelUniversidad Nacional Autonoma de Mexico, Instituto de Biologia, Circuito exterior s/n, Ciudad de Mexico, Ciudad de, Mexico, 4510, México.ORCID http://orcid.org/0000-0002-6977-7550
József GemlEnvironmental Microbiome Research Group, Research and Development Centre, Eszterházy Károly Catholic University, Leányka u. 8, Eger, 3300, Hungary.ORCID http://orcid.org/0000-0001-8745-0423
Soumya GhoshNatural and Medical Sciences Research Center, University of Nizwa, Nizwa, 616, Oman.ORCID http://orcid.org/0000-0002-4945-3516
Roberto GodoyInstituto de Ciencias Ambientales y Evolutivas, Universidad Austral de Chile, Isla Teja sn, Valdivia, 5090000, Chile.ORCID http://orcid.org/0000-0002-3719-3091
Daniyal GoharDepartment of Botany and Plant Pathology, Oregon State University, 2701 SW Campus Way, Corvallis, OR, 97331, USA.ORCID http://orcid.org/0000-0003-0312-1142
Marieka GryzenhoutDepartment of Genetics, Natural and Agricultural Sciences, University of the Free State, Bloemfontein, 9300, South Africa.
Ayad H HasanDepartment of Medical Microbiology, Faculty of Science and Health, Koya University, Koya, 44023, Kurdistan Region - F.R., Iraq.ORCID http://orcid.org/0000-0001-7280-2254
Amr H HashemBotany and Microbiology Department, Faculty of Science, Al-Azhar University, Nasr City, Cairo, 11884, Egypt.
Jacob Heilmann-ClausenCenter for Macroecology, Evolution and Climate, Globe institute, University of Copenhagen, Universitetsparken 15, Copenhagen, 2100, Denmark.ORCID http://orcid.org/0000-0003-4713-6004
Terry W HenkelDepartment of Biological Sciences, California State Polytechnic University, Humboldt, Arcata, CA, 95521, USA.ORCID http://orcid.org/0000-0001-9760-8837
Indrek HiiesaluInstitute of Ecology and Earth Sciences, University of Tartu, Juhan Liivi 2, Tartu, 50409, Estonia.
Inga HiiesaluDepartment of Botany, Institute of Ecology and Earth Sciences, University of Tartu, Juhan Liivi 2, Tartu, 50409, Estonia.ORCID http://orcid.org/0000-0002-5457-2376
Mahdieh S Hosseyni MoghaddamDepartment of Botany, Institute of Ecology and Earth Sciences, University of Tartu, Juhan Liivi 2, Tartu, 50409, Estonia.
Kevin D HydeDepartment of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh, 11495, Saudi Arabia.
Karina InostrozaBIOSFERA Research & Conservation, DS Can Mutxo, Sils, Girona, 17410, Spain.ORCID http://orcid.org/0000-0003-3026-6291
Khalil KarimanUWA School of Agriculture and Environment, The University of Western Australia, Perth, WA, 6009, Australia.ORCID http://orcid.org/0000-0002-2070-4713
Elina KarimullinaMicrobiology, Immunology and Infectious Diseases, University of Calgary, 3330 Hospital Drive NW, Calgary, Alberta, T2N 4N1, Canada.ORCID http://orcid.org/0000-0001-6459-2293
Sebastian Kepfer-RojasGeosciences and Natural Resource Management, University of Copenhagen, Rolighedsvej 23, Frederiksberg, 1958, Denmark.ORCID http://orcid.org/0000-0002-1681-2877
Abdul Nasir KhalidInstitute of Botany, University of the Punjab, Lahore, Punjab, 54320, Pakistan.
Darta KlavinaLatvian State Forest Research Insititute "Silava", Rīga str. 111, Salaspils, LV-2169, Latvia.ORCID http://orcid.org/0000-0002-1455-9062
Petr KohoutLaboratory of Microbial Ecology and Biogeography, Institute of Microbiology, Czech Academy of Science, Videnska 1083, Prague, 14220, Czechia.ORCID http://orcid.org/0000-0002-3985-2310
Yuri N Korotkovno affiliation, Tankhoi, Russia.ORCID http://orcid.org/0000-0001-8436-0887
John Y KupagmeMycology and Microbiology Center, University of Tartu, Juhan Liivi 2, Tartu, 50409, Estonia.ORCID http://orcid.org/0000-0002-9981-050X
Olavi KurinaChair of Biological Diversity and Nature Tourism, Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, F. R. Kreutzwaldi 5, Tartu, 51006, Estonia.ORCID http://orcid.org/0000-0002-4858-4629
Louis James LamitDepartment of Biology, Syracuse University, 107 College Place, Syracuse, NY, 13244, USA.ORCID http://orcid.org/0000-0002-0385-6010
Adebola Azeez LateefDepartment of Plant Biology, Faculty of Life Sciences, University of Ilorin, Ilorin, Kwara, 1515, Nigeria.
Njouonkou André LedouxDepartment of Plant Sciences, Faculty of Science, The University of Bamenda, Bambili, North-West Region, PO Box 39, Cameroon.ORCID http://orcid.org/0000-0001-9733-7248
Young Woon LimSchool of Biological Sciences, College of Natural Science, Institue of Biodiversity, Gwanak-ro 1, Seoul, 8826, South Korea.ORCID http://orcid.org/0000-0003-2864-3449
Jose G Maciá-VicenteDepartment of Marine Sciences and Applied Biology, University of Alicante, Alicante, 03080, Spain.
Kristaps MakovskisLatvian State Forest Research Insititute "Silava", Rīga str. 111, Salaspils, LV-2169, Latvia.ORCID http://orcid.org/0000-0003-4943-1912
Sebastián MartínezLaboratorio de Patología Vegetal, Instituto Nacional de Investigación Agropecuaria, Ruta 8, Km 281, Treinta y Tres, 33000, Uruguay.ORCID http://orcid.org/0000-0003-0455-5823
César MarínCentro de Investigación e Innovación para el Cambio Climático (CiiCC), Universidad Santo Tomás, Av. Ramón Picarte 1130, Valdivia, 5090000, Chile.ORCID http://orcid.org/0000-0002-2529-8929
Peter MeidlSoil Ecology, Free University Berlin, Berlin, 13357, Germany.
Peter E MortimerSoil Science, Stellenbosch University, PO BOX X1, Matieland, Stellenbosch, Western Cape, 7600, South Africa.
Sunil MundraDepartment of Biology, College of Sceince, United Arab Emirates University, Al Ain, Abu Dhabi, UAE.ORCID http://orcid.org/0000-0002-0535-118X
Victoria NaluyangeDepartment of Land Use Management, School of Agriculture, Veterinary Sciences and Technology, Masinde Muliro University of Science and Technology, Kakamega, 190-50100, Kenya.ORCID http://orcid.org/0000-0002-3990-9751
Tarquin NetherwayDepartment of Agroecology, Aarhus University, Forsøgsvej 1, Slagelse, 4200, Denmark.ORCID http://orcid.org/0000-0002-9049-9225
Kevin K NewshamBritish Antarctic Survey, Natural Environment Research Council, Madingley Road, Cambridge, CB3 0ET, UK.ORCID http://orcid.org/0000-0002-9108-0936
Eduardo NouhraCONICET-FCEFyN, National University of Córdoba, Av. Vélez Sarsfield 1666, Córdoba, Córdoba, X5016GCN, Argentina.ORCID http://orcid.org/0000-0002-7080-8211
Casper NyamukondiwaDepartment of Biological Sciences and Biotechnology, Botswana International University of Science and Technology, P. Bag 16, Palapye, Botswana.
Vincent NteziryayoFood Science and Technology, Faculty of Agriculture and Bio-engineering, University of Burundi, Avenue l'Unesco no 2, Bujumbura, Burundi, 2940, Burundi.ORCID http://orcid.org/0000-0002-0176-5780
Dennis M W OchienoDepartment of Biological Sciences, School of Natural Sciences, Masinde Muliro University of Science and Technology, Kakamega-Webuye Road, Kakamega, 254, Kenya.ORCID http://orcid.org/0000-0003-3985-9421
Jane OjaDepartment of Botany, Institute of Ecology and Earth Sciences, University of Tartu, Juhan Liivi 2, Tartu, 50409, Estonia.ORCID http://orcid.org/0000-0003-1446-2284
Vladimir G Onipchenkono affiliation, Moscow, Russia.ORCID http://orcid.org/0000-0002-1626-1171
Eveli OtsingMycology and Microbiology Center, University of Tartu, Juhan Liivi 2, Tartu, 50409, Estonia.ORCID http://orcid.org/0000-0001-7416-257X
Mustafa Nadhim OwaidDepartment of Environment, College of Applied Sciences-Hit, University Of Anbar, Hit, Anbar, Iraq.ORCID http://orcid.org/0000-0001-9005-4368
Meike PiepenbringMycology, Goethe University, Max-von-Laue-Str. 13, Frankfurt am Main, Hesse, 60438, Germany.ORCID http://orcid.org/0000-0002-7043-5769
Polina PochekutovaMycology and Microbiology Center, University of Tartu, Juhan Liivi 2, Tartu, 50409, Estonia.ORCID http://orcid.org/0009-0001-0453-9869
Maihyra Marina PomboDepartamento de Botânica, Divisão de Biodiversidade, Instituto Nacional de Pesquisa da Amazônia, Manaus, Amazonas, 69067-375, Brazil.ORCID http://orcid.org/0000-0002-0329-9736
Karin PritschResearch Unit Environmental Simulation, Helmholtz Zentrum München, Ingolstädter Landstraße 1, Neuherberg, 85764, Germany.ORCID http://orcid.org/0000-0001-6384-2473
Rasmus PuuseppMycology and Microbiology Center, University of Tartu, Juhan Liivi 2, Tartu, 50409, Estonia.ORCID http://orcid.org/0000-0002-0617-3776
Jaan PärnDepartment of Geography, Institute of Ecology and Earth Sciences, University of Tartu, Vanemuise 46, Tartu, 51003, Estonia.ORCID http://orcid.org/0000-0001-6507-8894
Kadri PõldmaaDepartment of Botany, Institute of Ecology and Earth Sciences, University of Tartu, Juhan Liivi 2, Tartu, 50409, Estonia.ORCID http://orcid.org/0000-0002-7936-2455
Saleh RahimlouDepartment of Plant Pathology and Environmental Microbiology, Pennsylvania State University, University Park, State College, PA, 16802, USA.ORCID http://orcid.org/0000-0003-0427-1329
Andrea C RinaldiDepartment of Biomedical Sciences, University of Cagliari, Cittadella Universitaria, Monserrato, CA, I09042, Italy.ORCID http://orcid.org/0000-0002-9352-1037
Oscar RojasFreshwater Biology Section, Department of Biology, University of Copenhagen, Universitetsparken 4, Copenhagen, 2100, Denmark.ORCID http://orcid.org/0000-0002-0176-9685
Tomas RoslinDepartment of Ecology, Swedish University of Agricultural Sciences (SLU), Ulls väg 18B, Uppsala, 75651, Sweden.ORCID http://orcid.org/0000-0002-2957-4791
Kadri RunnelDepartment of Zoology, Institute of Ecology and Earth Sciences, University of Tartu, Juhan Liivi 2, Tartu, 50409, Estonia.ORCID http://orcid.org/0000-0002-7308-3623
Elisabeth RähnChair of Silviculture and Forest Ecology, Institute of Forestry and Engineering, Estonian University of Life Sciences, F. R. Kreutzwaldi 5, Tartu, 51006, Estonia.
Malka SabaDepartment of Plant Sciences, Quaid-i-Azam University, Islamabad, 45320, Pakistan.ORCID http://orcid.org/0000-0001-7673-2345
Alessandro SaittaDepartment of Agricultural, Food and Forest Sciences, University of Palermo, Viale Delle Scienze, Palermo, 90123, Italy.
Talal Sabhan SalihDepartment of Medical Physics, College of Science, University of Mosul, Mosul, Iraq.ORCID http://orcid.org/0000-0001-8345-6131
Joosep SarapuuEstonian Museum of Natural History, Lai 29a, Tallinn, 10133, Estonia.ORCID http://orcid.org/0000-0002-4805-5612
Eduard SerranoBIOSFERA Research & Conservation, DS Can Mutxo, Sils, Girona, 17410, Spain.ORCID http://orcid.org/0000-0001-9082-473X
Oscar SerranoMarine ecology, Centre for Advanced Studies of Blanes, CSIC, Carrer Accés Cala Sant Francesc, 14, Blanes, Girona, 17300, Spain.ORCID http://orcid.org/0000-0002-5973-0046
Dipon SharmahDepartment of Botany, Jawaharlal Nehru Rajkeeya Mahavidyalaya, Pondicherry University, Port Blair, Andaman and Nicobar Islands, 744101, India.ORCID http://orcid.org/0000-0002-5579-3103
Cathy SharpNatural History Museum of Zimbabwe, cnr Park Road & Leopold Takawira Avenue Centenary Park, Bulawayo, Zimbabwe.ORCID http://orcid.org/0009-0003-4985-1543
Maria W Skalska-TuomiDepartment of Geographical and Historical Studies, University of Eastern Finland, Yliopistokatu 7, Joensuu, 80101, Finland.ORCID http://orcid.org/0000-0002-7154-5177
Kassim Issifou TchanDepartment of Forestry and Wildlife Management, Science agronomique Kigani-Dada de Kpéssou, Private Agricultural University, Parakou, Benin.ORCID http://orcid.org/0000-0002-8572-3405
Camille TruongRoyal Botanic Gardens Victoria, Melbourne, VIC, 3004, Australia.ORCID http://orcid.org/0000-0002-8510-1761
Helga van der MerweSouth African Environmental Observation Network, Arid Lands Node, 97 Memorial Road, Kimberley, 8036, South Africa.ORCID http://orcid.org/0000-0002-7677-5123
Linda L P Vanié-LéaboUFR Biosciences, Université Félix Houphouet-Boigny, Abidjan, BPV34, Côte d'Ivoire.ORCID http://orcid.org/0000-0001-6504-3456
Aida M Vasco-PalaciosGrupo BioMicro, Escuela de Microbiología, Universidad de Antioquia UdeA, Calle 70 No. 52-2, Medellin, 50010, Colombia.ORCID http://orcid.org/0000-0003-0539-9711
Annemieke VerbekenDepartment of Biology, Ghent University, K.L. Ledeganckstraat 35, Gent, 9000, Belgium.
Lukáš VlkDepartment of Invasion Ecology, Institute of Botany of the Czech Academy of Sciences, Zámek 1, Průhonice, 25243, Czechia.ORCID http://orcid.org/0000-0001-9201-8715
Nalin N WijayawardeneCenter for Yunnan Plateau Biological Resources, Protection and Utilization & Yunnan International Joint Laboratory of Fungal Sustainable Utilization in South and Southeast Asia, Biology and Food Engineering, Qujing Normal University, Qujing, Yunnan, 655011, China.ORCID http://orcid.org/0000-0003-0522-5498
Jennifer L WoodDepartment of Microbiology, Anatomy, Physiology and Pharmacology, La Trobe University, Bundoora, VIC, 3086, Australia.ORCID http://orcid.org/0000-0002-7313-5681
W A Erandi YasanthikaCenter of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai, 57100, Thailand.ORCID http://orcid.org/0000-0002-3757-3801
Nourou S YorouTropical Mycology and Plant-Soil fungi Interactions (MyTIPS), Faculty of Agronomy, University of Parakou, Parakou, Benin.ORCID http://orcid.org/0000-0001-6997-811X
Geoffrey ZahnApplied Science Department, William & Mary, 540 Landrum Drive, Williamsburg, VA, 23185, USA.ORCID http://orcid.org/0000-0002-8691-692X
Irma ZetturNatural History Museum, University of Tartu, Vanemuise 46, Tartu, 51003, Estonia.
Laura ZucconiDepartment of Ecological and Biological Sciences, University of Tuscia, Largo dell'Università, Viterbo, 01100, Italy.ORCID http://orcid.org/0000-0001-9793-2303
Urmas KõljalgDepartment of Botany, Institute of Ecology and Earth Sciences, University of Tartu, Juhan Liivi 2, Tartu, 50409, Estonia.ORCID http://orcid.org/0000-0002-5171-1668
Leho TedersooDepartment of Zoology, College of Science, King Saud University, P.O. Box 2455, Riyadh, 11451, Saudi Arabia. leho.tedersoo@ut.ee.ORCID http://orcid.org/0000-0002-1635-1249
Funding
The Estonian Ministry of Education and Research Center of Excellence AgroCropFuture "Agroecology and new crops in future climates", TK200The Estonian Research Council PRG1789The Estonian Research Council PRG632The European Research Council (ERC) 101200758 (PhylFun ERC-2024-ADG)The Swedish Foundation for Strategic Environmental Research MISTRA BioPathThe Swedish Research Council 2024-04303
Abstract
Soil eukaryotes, including fungi, protists, plants, and animals, are central to biosphere functioning and resilience. The Global Standardised Soil Eukaryome Dataset (GloSED) is the first dataset encompassing the entire spectrum of soil eukaryotes, covering 4,063 sampling sites in 121 countries on all continents, revealing nearly one million operational taxonomic units. All samples were collected and analysed using a standardised protocol minimizing technical biases. Long-read sequencing of full-length ITS and 18S-V9 regions provide broad taxonomic coverage and high-resolution identification supported by specialist curation of "dark taxa". A rigorous bioinformatic processing ensures against homopolymer errors, PCR-mediated chimeras, and index switching providing high data quality. The dataset is supported by raw sequences and an open-source containerised workflow for reproducible analyses. The samples are accompanied by land-cover description and directly measured soil pH, δ
Indexed as
EukaryotaSoilSoil MicrobiologySequence Analysis, DNASoil
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