Evidence map›Paper›PMID 42090279›Full record

ArticleInvestigative ophthalmology & visual science2026

High-Dose Sucralose Is Associated With Lacrimal Circadian Remodeling, Reduced Immunometabolic Signatures, and Reduced Tear Secretion.

Di Qi, Tingting Yang, Xiaoting Pei, Dingli Lu, Shenzhen Huang, Liu Yang, Jingwen Yang, Shuting Xuan, Mengru Ba, Wenxiao Zhang and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Investigative ophthalmology & visual science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Di QiHenan Eye Hospital and Henan Key Laboratory of Ophthalmology and Visual Science, Henan Eye Hospital, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, People's Hospital of Henan University, Zhengzhou, Henan, China.
Tingting YangDepartment of Ophthalmology, People's Hospital of Henan University, Henan Provincial People's Hospital, Zhengzhou, Henan, China.
Xiaoting PeiHenan Eye Hospital and Henan Key Laboratory of Ophthalmology and Visual Science, Henan Eye Hospital, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, People's Hospital of Henan University, Zhengzhou, Henan, China.
Dingli LuHenan Eye Hospital and Henan Key Laboratory of Ophthalmology and Visual Science, Henan Eye Hospital, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, People's Hospital of Henan University, Zhengzhou, Henan, China.
Shenzhen HuangHenan Eye Hospital and Henan Key Laboratory of Ophthalmology and Visual Science, Henan Eye Hospital, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, People's Hospital of Henan University, Zhengzhou, Henan, China.
Liu YangHenan Eye Hospital and Henan Key Laboratory of Ophthalmology and Visual Science, Henan Eye Hospital, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, People's Hospital of Henan University, Zhengzhou, Henan, China.
Jingwen YangHenan Eye Hospital and Henan Key Laboratory of Ophthalmology and Visual Science, Henan Eye Hospital, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, People's Hospital of Henan University, Zhengzhou, Henan, China.
Shuting XuanDepartment of Ophthalmology, People's Hospital of Henan University, Henan Provincial People's Hospital, Zhengzhou, Henan, China.
Mengru BaDepartment of Ophthalmology, People's Hospital of Zhengzhou University, Henan Provincial People's Hospital, Zhengzhou, Henan, China.
Wenxiao ZhangDepartment of Ophthalmology, People's Hospital of Zhengzhou University, Henan Provincial People's Hospital, Zhengzhou, Henan, China.
Xiaohui LiuThe First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Shufan YuDepartment of Ophthalmology, People's Hospital of Henan University, Henan Provincial People's Hospital, Zhengzhou, Henan, China.
Zhijie LiHenan Eye Hospital and Henan Key Laboratory of Ophthalmology and Visual Science, Henan Eye Hospital, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, People's Hospital of Henan University, Zhengzhou, Henan, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Purpose: To determine whether high-dose sucralose (SUC) exposure alters circadian organization of the mouse extraorbital lacrimal gland, immunometabolic pathways, and tear film function using multi-omics and functional assays. Methods: Male C57BL/6J mice received SUC (0.72 mg/mL in drinking water) or water under a 12:12 hour light-dark cycle for 30 days. Extraorbital lacrimal glands were collected every 3 hours across 24 hours (n = 3/time point/group) for RNA sequencing and 4D-data-independent acquisition proteomics. Rhythms were identified with JTK_CYCLE, differential expression with DESeq2/MSstats followed by Kyoto Encyclopedia of Genes and Genomes pathway analysis. Oil Red O and CD4/CD8 immunohistochemistry assessed lipid and immune signatures. Tear secretion, tear film breakup time, and corneal fluorescein staining were measured at ZT0, ZT6, ZT12, and ZT18. Results: SUC increased rhythmic transcripts (3074 to 4600) and proteins (378 to 984), redistributing acrophases (transcript peaks shifted toward ZT3-ZT6; protein peaks toward ZT21-ZT24), indicating clock phase remodeling. Both omics layers converged at the pathway level, showing downshifts in metabolic and immune programs, including complement- and T-cell-related signatures. SUC elevated water intake and body weight without altering glucose tolerance. Functionally, SUC-treated mice showed reduced stimulated tear secretion, whereas tear film breakup time and corneal fluorescein staining did not differ significantly. Conclusions: Under this high-exposure paradigm, SUC was associated with lacrimal circadian remodeling, reduced metabolic and immune pathway signatures, and lower stimulated tear secretion, identifying candidate pathways for mechanistic testing without establishing direct causality.

Indexed as

Circadian RhythmDry Eye SyndromesLacrimal ApparatusSucroseSweetening AgentsTearsAnimalsMaleMiceMice, Inbred C57BLSucroseSweetening Agentstrichlorosucrose

Identifiers

PMID42090279
PMCPMC13170728

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.