Evidence map›Paper›PMID 42092004›Full record

ArticleScientific reports2026

Collagen hybridizing peptide to target in vivo misfolded collagen in OI zebrafish.

Francesca Tonelli, Carla Aresi, Cecilia Masiero, Roberta Gioia, Tian Morrison, Antonio Rossi, S Michael Yu, Antonella Forlino

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Francesca TonelliDepartment of Molecular Medicine, Biochemistry Unit, University of Pavia, Via Taramelli 3/b, Pavia, 27100, Italy.
Carla AresiDepartment of Molecular Medicine, Biochemistry Unit, University of Pavia, Via Taramelli 3/b, Pavia, 27100, Italy.
Cecilia MasieroDepartment of Molecular Medicine, Biochemistry Unit, University of Pavia, Via Taramelli 3/b, Pavia, 27100, Italy.
Roberta GioiaBiomedical Imaging Laboratory, Centro Grandi Strumenti, University of Pavia, Pavia, Italy.
Tian MorrisonDepartment of Biomedical Engineering, University of Utah, Salt Lake City, UT, USA.
Antonio RossiDepartment of Molecular Medicine, Biochemistry Unit, University of Pavia, Via Taramelli 3/b, Pavia, 27100, Italy.
S Michael YuDepartment of Biomedical Engineering, University of Utah, Salt Lake City, UT, USA.
Antonella ForlinoDepartment of Molecular Medicine, Biochemistry Unit, University of Pavia, Via Taramelli 3/b, Pavia, 27100, Italy. antonella.forlino@unipv.it.

Funding

Ministero dell'Istruzione, dell'Università e della Ricerca Dipartimenti di Eccellenza (2023-2027)National Institute of Health, USA R01AR084689Regione Lombardia regionallawn°9/2020, resolutionn°3776/202000
6 · The paper itself

Abstract

Osteogenesis imperfecta (OI) is a heritable connective tissue disorder characterized by defects in collagen I, leading to increased bone fragility and turnover. The zebrafish Chihuahua (Chi/+), carrying a p.(Gly736Asp) substitution in the α1(I) chain, recapitulates key features of human OI, including the presence of misfolded collagen I in extracellular matrix. In this study, we evaluated the specificity of a fluorescently labeled collagen hybridizing peptide (Cy5-CHP) for detecting in vivo misfolded collagen I in adult zebrafish. The CHP or the scrambled control peptides were injected intraperitoneally into WT and Chi/+ zebrafish, and average radiant efficiency was quantified at 24 and 72 h post-injection (hpi) in the vertebral column, cranium and heart, representing collagen I-rich organs, as well as the brain, a collagen I-poor tissue. Our results revealed Cy5-CHP specific binding to collagen I-rich tissues in Chi/+ zebrafish at 24 hpi, with minimal signal in the brain, whereas low signal was detectable across all tissues in WT. Cy5-CHP fluorescence was reduced by 72 hpi in all samples, consistent with the low binding affinity of the peptide. Histological analyses confirmed the co-localization of Cy5-CHP with collagen fibers in the endplates of Chi/+ vertebrae at 24 hpi. These findings provide the first in vivo evidence in zebrafish that Cy5-CHP selectively binds misfolded collagen, showing preferential accumulation in collagen I-rich tissues in OI. Overall, the results support the use of Cy5-CHP as a sensitive tool for detecting pathologic collagen and as a potential platform for tissue-targeted drug delivery in OI and other collagen-related disorders.

Indexed as

CollagenCollagen Type IOsteogenesis ImperfectaPeptidesAnimalsCarbocyaninesDisease Models, AnimalHumansProtein FoldingZebrafishCarbocyaninesCollagenCollagen Type IPeptidesBoneCHPMisfolded collagenOsteogenesis imperfectaZebrafish

Identifiers

PMID42092004
PMCPMC13338455

What Socratic holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.