Evidence map›Paper›PMID 42096907›Full record

ArticlePoultry science2026

Immunocastration with recombinant GnRH6-CRM197 fusion protein improves reproductive suppression and meat tenderness in roosters.

Mengxian Li, Kejian Han, Dongyuan Fu, Rui Zhang, Xiangyang Jiang, Juntai Lu, Yuxin Yan, Junhong Li, Ya Liu, Yunsheng Li and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Poultry science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Mengxian LiDepartment of Basic Veterinary, College of Veterinary Medicine, Anhui Agricultural University, 130 Changjiang West Road, Hefei, Anhui 230036, China.
Kejian HanDepartment of Basic Veterinary, College of Veterinary Medicine, Anhui Agricultural University, 130 Changjiang West Road, Hefei, Anhui 230036, China.
Dongyuan FuDepartment of Basic Veterinary, College of Veterinary Medicine, Anhui Agricultural University, 130 Changjiang West Road, Hefei, Anhui 230036, China.
Rui ZhangDepartment of Basic Veterinary, College of Veterinary Medicine, Anhui Agricultural University, 130 Changjiang West Road, Hefei, Anhui 230036, China.
Xiangyang JiangDepartment of Basic Veterinary, College of Veterinary Medicine, Anhui Agricultural University, 130 Changjiang West Road, Hefei, Anhui 230036, China.
Juntai LuDepartment of Basic Veterinary, College of Veterinary Medicine, Anhui Agricultural University, 130 Changjiang West Road, Hefei, Anhui 230036, China.
Yuxin YanDepartment of Basic Veterinary, College of Veterinary Medicine, Anhui Agricultural University, 130 Changjiang West Road, Hefei, Anhui 230036, China.
Junhong LiDepartment of Basic Veterinary, College of Veterinary Medicine, Anhui Agricultural University, 130 Changjiang West Road, Hefei, Anhui 230036, China.
Ya LiuDepartment of Basic Veterinary, College of Veterinary Medicine, Anhui Agricultural University, 130 Changjiang West Road, Hefei, Anhui 230036, China; Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China.
Yunsheng LiDepartment of Basic Veterinary, College of Veterinary Medicine, Anhui Agricultural University, 130 Changjiang West Road, Hefei, Anhui 230036, China; Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China.
Tong YuDepartment of Basic Veterinary, College of Veterinary Medicine, Anhui Agricultural University, 130 Changjiang West Road, Hefei, Anhui 230036, China; Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China.
Hongwei DuanDepartment of Basic Veterinary, College of Veterinary Medicine, Anhui Agricultural University, 130 Changjiang West Road, Hefei, Anhui 230036, China; Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China.
Fugui FangDepartment of Basic Veterinary, College of Veterinary Medicine, Anhui Agricultural University, 130 Changjiang West Road, Hefei, Anhui 230036, China; Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China. Electronic address: fgfang@ahau.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study evaluated the potential of the recombinant GnRH6-CRM197 fusion protein as an immunocastration vaccine for roosters. Forty 4-week-old roosters were divided into two groups: the immune group and the control group that received a saline injection. The vaccine (1 mg/mL) was administered at 4 and 8 weeks of age. Body weights and blood samples were collected at 4, 8, 12, 16, and 20 weeks of age, with culling performed at 20 weeks. Serum anti-GnRH antibody titers and testosterone concentrations were measured. The testicles were collected for morphological analysis, while pectoral and thigh muscle samples were assessed for muscle fiber characteristics, intramuscular fat, and meat quality indicators. The results demonstrated that the immune group exhibited a significant induction of anti-GnRH antibodies (P < 0.05). Vaccination significantly suppressed comb and wattle development (persisting until week 16) and markedly reduced testis size, weight, and serum testosterone levels (P < 0.05). Histological examination confirmed a severe reduction in germ cell numbers at all developmental stages. Regarding the quality of the meat, the tenderness of the breast exhibited a significant enhancement in the group that had been immunized. However, no significant differences were observed among groups in live weight, carcass yield, muscle pH, meat color, or intramuscular fat content (P > 0.05). In summary, the GnRH6-CRM197 vaccine effectively suppresses reproductive function in roosters while enhancing meat tenderness, demonstrating potential for application as an immunocastration agent in poultry without adversely affecting key production indicators.

Indexed as

Bacterial ProteinsChickensGonadotropin-Releasing HormoneMeatOrchiectomyReproductionAnimalsMaleRecombinant Fusion ProteinsTestisTestosteroneBacterial ProteinsGonadotropin-Releasing HormoneRecombinant Fusion ProteinsTestosteroneRecombinant GnRH6-CRM197Reproductive functionRooster

Identifiers

PMID42096907
PMCPMC13157140

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.