A high throughput assay to identify modulators of Death Receptor 3 (DR3).

Emery Smith, Joshua K Parker, Izan Gonzalez Fayos, Nicholas R Stan, Louis Scampavia, Thomas Kodadek, Timothy P Spicer

Abstract read

In one paragraph

Article in SLAS discovery : advancing life sciences R & D, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Emery SmithThe Herbert Wertheim UF Scripps Institute for Biomedical Innovation & Technology, Department of Molecular Medicine, Jupiter, FL 33458, USA.

Joshua K ParkerThe Herbert Wertheim UF Scripps Institute for Biomedical Innovation & Technology, Department of Chemistry, Jupiter, FL 33458, USA.

Izan Gonzalez FayosThe Herbert Wertheim UF Scripps Institute for Biomedical Innovation & Technology, Department of Molecular Medicine, Jupiter, FL 33458, USA.

Nicholas R StanThe Herbert Wertheim UF Scripps Institute for Biomedical Innovation & Technology, Department of Chemistry, Jupiter, FL 33458, USA.

Louis ScampaviaThe Herbert Wertheim UF Scripps Institute for Biomedical Innovation & Technology, Department of Molecular Medicine, Jupiter, FL 33458, USA.

Thomas KodadekThe Herbert Wertheim UF Scripps Institute for Biomedical Innovation & Technology, Department of Chemistry, Jupiter, FL 33458, USA.

Timothy P SpicerThe Herbert Wertheim UF Scripps Institute for Biomedical Innovation & Technology, Department of Molecular Medicine, Jupiter, FL 33458, USA. Electronic address: spicert@ufl.edu.

Funding

University of Florida Health Cancer Center Support GrantP30CA247796 · NCI · UNIVERSITY OF FLORIDA · PI JI-HYUN LEE · 2023 to 2026

$10.9M

NCI NIH HHS P30 CA247796

6 · The paper itself

Abstract

Association of the TNF-like cytokine 1A (TL1A) with Death Receptor 3 (DR3) triggers a signaling cascade in T cells that results in the increased AP-1- and NF-κB-mediated expression of inflammation-promoting gene products. Dysregulation of TL1A/DR3 signaling is a major contributor to many autoimmune diseases, cancers, and other conditions yet there is a dearth of small molecule regulators of this pathway. Here we report a cell-based high throughput screen of 8000 compounds of compounds that yielded 12 antagonists but no agonists of this pathway.

Indexed as

Drug DiscoveryHigh-Throughput Screening AssaysReceptors, Tumor Necrosis Factor, Member 25HumansNF-kappa BSignal TransductionSmall Molecule LibrariesT-LymphocytesTumor Necrosis Factor Ligand Superfamily Member 15NF-kappa BReceptors, Tumor Necrosis Factor, Member 25Small Molecule LibrariesTNFRSF25 protein, humanTNFSF15 protein, humanTumor Necrosis Factor Ligand Superfamily Member 15AgonistAntagonistCell-basedDeath receptorInflammationNF-KB

Identifiers

PMID42097330

DOI10.1016/j.slasd.2026.100312

PMCPMC13557041

What Socratic holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.