Evidence map›Paper›PMID 42104184›Full record

ArticleLasers in medical science2026

Optimisation of low-level light therapy (lllt) parameters and evaluation of its immunomodulatory effect on fibroblasts.

Anna Ścisłowska-Czarnecka, Amelia Lizak, Aleksandra Matuła, Aneta Bac, Joanna Homa, Magdalena Chadzińska, Beata Stenka

Abstract read
In one paragraph

Article in Lasers in medical science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Anna Ścisłowska-CzarneckaDepartment of Applied Cosmetology, University of Physical Culture, Kraków, Poland. anna.scislowska@awf.krakow.pl.
Amelia LizakDepartment of Applied Cosmetology, University of Physical Culture, Kraków, Poland.
Aleksandra MatułaDepartment of Applied Cosmetology, University of Physical Culture, Kraków, Poland.
Aneta BacDepartment of Applied Cosmetology, University of Physical Culture, Kraków, Poland.
Joanna HomaDepartment of Evolutionary Immunology, Institute of Zoology and Biomedical Research, Jagiellonian University, Kraków, Poland.
Magdalena ChadzińskaDepartment of Evolutionary Immunology, Institute of Zoology and Biomedical Research, Jagiellonian University, Kraków, Poland.
Beata StenkaDepartment of Applied Cosmetology, University of Physical Education and Sport, Gdańsk, Poland.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Low-level laser therapy (LLLT) is being increasingly applied in regenerative medicine due to its ability to modulate cellular metabolism and support repair processes. However, the effect of various exposure parameters on fibroblast activity still does not remain fully understood. The study aim was to evaluate the effect of selected LLLT parameters (808 nm, 100 and 200 mW, 2 and 10 J/cm², pulsed beam) on the biological activity of fibroblasts in vitro. Fibroblasts were exposed to the established exposure parameters, and then the number of adherent and metabolically active cells, their migration, adenylate kinase (AK) as well as nitric oxide (NO) levels, cytokine secretion, metalloproteinase (MMP-2, MMP-9) activity, and oxidative-antioxidant potential were assessed. The most favourable effects were achieved with 200 mW; 2 J/cm² and 100 m; 10 J/cm² exposure parameters. In these conditions, increased number of adherent and metabolically active cells, and elevated migration were observed. Moreover, in this condition decreased AK release and NO secretion were found. Furthermore, LLLT reduced in fibroblasts interleukin 6 (IL-6) and interleukin 8 (IL-8) secretion as well as affected the activity of metalloproteinases, what corresponded with subsequent wound healing process. Additionally, a beneficial effect on maintaining the oxidative-antioxidant balance in fibroblasts was noted. In summary, the effects of LLLT on fibroblasts depend on the applied irradiation parameters, and appropriately selected LLLT treatment (200 mW; 2 J/cm² and 100 mW; 10 J/cm²,) stabilise fibroblast metabolism, limits their proinflammatory response and promotes extracellular matrix remodelling (ECM). These results confirm the potential of LLLT as an effective method supporting regenerative processes in the treatment of tissue damage.

Indexed as

FibroblastsLow-Level Light TherapyAdenylate KinaseAntioxidantsCell MovementCells, CulturedCytokinesHumansInterleukin-6Interleukin-8Matrix Metalloproteinase 2Matrix Metalloproteinase 9Nitric OxideWound HealingAdenylate KinaseAntioxidantsCytokinesInterleukin-6Interleukin-8Matrix Metalloproteinase 2Matrix Metalloproteinase 9Nitric OxideCytokinesFibroblastsLLLTMMPOxidative-antioxidant potential

Identifiers

PMID42104184
PMCPMC13156211

What Socratic holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.