ArticleInfectious diseases of poverty2026
Comparative evaluation of Sm1-7-qPCR systems incorporated with Maxwell kit-based and NaOH-based DNA extraction methods for the detection of Schistosoma mansoni infection.
Article in Infectious diseases of poverty, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe World Health Organization's latest roadmap for neglected tropical diseases (NTDs) 2021-2030 has highlighted diagnostics as one of four focus areas to achieve the targets set for 2030. Molecular diagnostic tests, such as quantitative real-time PCR (qPCR), are valuable for diagnosing NTDs, including schistosomiasis. This study aimed to comparatively evaluate two Sm1-7-qPCR systems incorporating different DNA extraction approaches for the detection of Schistosoma mansoni infection.
methodsTwo copro-DNA-qPCR systems were developed: a Maxwell kit-based copro-DNA-Sm1-7-qPCR system (Sm1-7-qPCR-S1) and a NaOH-based copro-DNA-Sm1-7-qPCR system (Sm1-7-qPCR-S2). Both systems were tested on faecal samples collected from schistosomiasis endemic and non-endemic areas in Uganda (n = 482). A composite reference based on positive results of either the Kato-Katz (KK) or qPCR assays was used for calibration. The S. mansoni positivity rates were analyzed based on the results of different diagnostics. Differences in DNA concentration and cycle threshold (Ct) values from the two copro-DNA-qPCR systems were compared (Wilcoxon signed-rank test).
resultsThe overall S. mansoni positivity rate was 52.9% (229/433) according to the composite reference, whereas the KK, Sm1-7-qPCR-S1 and Sm1-7-qPCR-S2 recorded positivity rates of 36.7% (159/433), 43.9% (190/433), and 47.6% (206/433), respectively. Sm1-7-qPCR-S2 demonstrated a sensitivity of 90.0%, outperforming the KK and Sm1-7-qPCR-S1 with sensitivities of 69.4% and 83.0%, respectively (P < 0.0001 and P = 0.015, respectively, χ
conclusionsThis study highlights that DNA samples extracted from both systems are suitable for robust qPCR amplification. The Sm1-7-qPCR-S2 system offers several advantages over the Sm1-7-qPCR-S1 system, including improved sensitivity, field accessibility, and lower cost. With further refinement, the Sm1-7-qPCR-S2 system could be integrated into the existing schistosomiasis surveillance network to aid the elimination of the disease.
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.