ArticlePoultry science2026
Aging, inflammation, and oxidative stress converge on semen quality in Oravka roosters.
Article in Poultry science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Across one year of ageing, we characterized how semen quality deteriorates in Oravka roosters, an endangered Slovak chicken breed. Ejaculates were collected from the same mature males at approximately 62.5 weeks (Year 1) and 114.5 weeks (Year 2). We combined computer-assisted sperm analysis (CASA) of motility and kinematics with assessments of plasma membrane and acrosome integrity, phosphatidylserine externalization, membrane lipid organization, mitochondrial membrane potential, ATP metabolism, DNA integrity, oxidative status, seminal plasma biochemistry, antibacterial proteins, cytokines, immunoglobulins, Gram-negative and total aerobic bacterial loads, endotoxin and culturable seminal microbiota composition. Year 2 samples showed consistent declines in total and progressive motility, velocities and linearity, together with reduced membrane integrity and acrosome-intact fractions and higher Annexin V-, merocyanine- and propidium iodide-positive signals, indicating progressive membrane destabilization. Older ejaculates also exhibited mitochondrial depolarization, lower ATP, increased DNA fragmentation, higher reactive oxygen species production, increased lipid and protein oxidation and reduced total antioxidant capacity. Biochemically, semen from older roosters displayed lower pH and bicarbonate, increased osmolarity, sodium/potassium imbalance, and reduced calcium/magnesium, defining a less permissive extracellular milieu. Bacteriologically, Year 2 ejaculates showed higher Gram-negative and aerobic loads, elevated endotoxin and a shift from lactic acid-dominated communities to Escherichia coli- and Pseudomonas-rich profiles, together with reduced antibacterial proteins and a more pro-inflammatory cytokine pattern. Collectively, these data indicate that microbial and oxidative-inflammatory drift, membrane remodeling, mitochondrial dysfunction and biochemical imbalance jointly drive the age-linked decline in Oravka semen quality, and provide baseline evidence for selecting the age window and pre-collection management conditions for future conservation-oriented semen collection in this breed.
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.