ArticleAnimal bioscience2026
Supplementation of the freezing extender with glutathione peroxidase improves the post-thaw quality of ram semen by enhancing antioxidant capacity, regulating metabolic enzyme activity, and modulating proteome expression.
Article in Animal bioscience, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
objectiveThe present study was conducted to determine effects of different concentrations of glutathione peroxidase (GSH-Px) on sperm motility and viability, structural integrity, antioxidant capacity, and metabolic enzyme activity after freezing semen of sheep and to evaluate the expression of differential proteins.
methodsSemen samples were collected from six Dorper rams, pooled, and subsequently divided into four groups. GSH-Px was added to the cryoprotectant solution at concentrations of 0, 0.8, 1.6, and 3.2 units per liter, respectively. Following thawing, sperm motility, viability, and kinematic parameters were assessed, along with acrosomal and plasma membrane integrity, DNA integrity, mitochondrial activity, antioxidant status, metabolic enzyme activity, and proteomic profiles.
resultsAdding 1.6 U/L GSH-Px to the freezing extender significantly enhanced the motility and viability of sperm after freeze-thaw, as well as the integrity of the acrosome, plasma membrane and DNA. The activities of total antioxidant capacity (T-AOC), superoxide dismutase and catalase were significantly increased. The oxidative stress marker malondialdehyde was significantly reduced, and the activities of metabolic enzymes including lactate dehydrogenase, aspartate aminotransferase, and alanine aminotransferase were also decreased. Proteomic analysis identified 150 differentially expressed proteins. Western blotting demonstrated the increase G1 to S Phase Transition 1, ribosomal protein L11, and downregulated annexin A2 expression.
conclusionSupplementing the cryopreservation extender with 1.6 U/L GSH-Px significantly improves post-thaw ovine sperm quality, as evidenced by enhanced structural integrity, elevated T-AOC, and attenuated oxidative stress-induced damage.
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