ArticleMethods in molecular biology (Clifton, N.J.)2026
Virus-Induced NLR-Silencing Library-Based Screening to Identify Antiviral NLR Genes.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Traditional genetic methods for identifying disease-resistant nucleotide binding and leucine-rich repeat-containing receptors (NLRs) proteins are often laborious and time-consuming. To streamline the screening process, we employed a pre-constructed combinatorial Virus-Induced Gene Silencing (VIGS) library. This library targets 301 NbNLR genes by integrating specific fragments from 4 to 6 distinct NLRs into a single VIGS vector, generating 55 VIGS combination (VIGS-com). This chapter presents an efficient method based on tobacco rattle virus (TRV)-mediated VIGS for rapidly screening NLR genes that confer resistance to plant virus infection in Nicotiana benthamiana, using turnip mosaic virus (TuMV) as a case. This approach enables rapid and systematic screening of key disease-resistant NLR receptors in antiviral plant immunity.
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