ArticleRapid communications in mass spectrometry : RCM2026
Enhancing IR-MALDESI MSI Spatial Resolution Through Beam Constriction With a Ring-Actuated Iris.
Article in Rapid communications in mass spectrometry : RCM, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
rationaleInfrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) mass spectrometry imaging (MSI) enables label-free, spatially resolved molecular analysis of biological tissues under ambient conditions. While improving spatial resolution is important for mapping fine tissue structures, mid-infrared (IR) lasers are diffraction limited to laser spot sizes greater than ~5 μm, necessitating creative approaches to enhance spatial detail for subcellular MSI.
methodsAn adjustable, ring-actuated iris combined with a reflective objective was integrated into the IR-MALDESI MSI platform, replacing the conventional beam expander and collimator assembly. Laser beam diameter on target was systematically varied, and ion abundances were measured using high-resolution mass spectrometry. Normalized ion abundances were fitted to a linear model as a function of beam diameter to predict signal at increasing spatial resolutions.
resultsIon abundance decreased linearly with decreasing laser beam diameter across the tested range. Linear regression of normalized ion abundances enabled prediction of signal levels at progressively higher spatial resolutions. The tunable aperture provided stable beam control and reproducible ablation, allowing quantitative assessment of sensitivity trade-offs associated with reduced laser spot size.
conclusionsControlled beam restriction using an adjustable iris enables fine tuning of spatial resolution in IR-MALDESI MSI by deliberately discarding a large fraction of high laser power while maintaining measurable ion abundance. Far-field diffraction and tissue ablation thresholds confine material removal beyond simple geometric scaling, and linear modeling of ion abundance versus beam diameter enables quantitative prediction of signal loss at high spatial resolution.
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.