Evidence map›Paper›PMID 42140199›Full record

ArticleStem cell reports2026

Developmental dynamics of skeletal muscle can be recapitulated in vitro from pig embryonic stem cells.

Jinsol Jeong, Kwang-Hwan Choi, Yelim Ahn, Seokjong Lee, Jumi Kang, Beom Seok Choo, Dong-Wook Kim, Dong-Kyung Lee, Chang-Kyu Lee

Abstract read
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Article in Stem cell reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Jinsol JeongDepartment of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute of Agriculture and Life Science, Seoul National University, Seoul 08826, Korea.
Kwang-Hwan ChoiResearch and Development Center, Space F corporation, Hwaseong, Gyeonggi-do 18471, Korea.
Yelim AhnDepartment of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute of Agriculture and Life Science, Seoul National University, Seoul 08826, Korea.
Seokjong LeeDepartment of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute of Agriculture and Life Science, Seoul National University, Seoul 08826, Korea.
Jumi KangDepartment of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute of Agriculture and Life Science, Seoul National University, Seoul 08826, Korea.
Beom Seok ChooDepartment of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute of Agriculture and Life Science, Seoul National University, Seoul 08826, Korea.
Dong-Wook KimDepartment of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute of Agriculture and Life Science, Seoul National University, Seoul 08826, Korea.
Dong-Kyung LeeResearch and Development Center, Space F corporation, Hwaseong, Gyeonggi-do 18471, Korea.
Chang-Kyu LeeDepartment of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute of Agriculture and Life Science, Seoul National University, Seoul 08826, Korea; Institute of Green Bio Science and Technology, Seoul National University, Pyeongchang, Gangwon-do 25354, Korea. Electronic address: leeck@snu.ac.kr.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Given the value of pigs as a large-animal model bridging rodents and humans, a porcine in vitro system for skeletal muscle development is a prerequisite for advancing both fundamental and translational research. Here, we establish a stepwise and high-resolution in vitro fate-map of pig skeletal muscle development from embryonic stem cells (ESCs). Single-cell analysis revealed three "myogenic states" faithfully recapitulating the conserved in vivo developmental trajectory: (1) specification of posterior presomitic mesoderm (PSM), (2) PSM patterning and somitogenesis, and (3) myogenesis from somite. Furthermore, we uncover porcine molecular signatures by identifying CDH15 and ADAM22 as markers for pig ESC-derived myogenic progenitors capable of generating multinucleated myotubes. Altogether, we present a comprehensive in vitro roadmap of pig skeletal muscle development by constructing previously uncharacterized developmental dynamics as a continuum. These findings offer a powerful resource for comparative developmental biology and translational preclinical research.

Indexed as

Embryonic Stem CellsMuscle DevelopmentMuscle, SkeletalAnimalsCadherinsCell DifferentiationGene Expression Regulation, DevelopmentalMesodermSingle-Cell AnalysisSomitesSwineCadherinsdevelopmentESCfate-mappingmusclePigsingle-cell RNA sequencing

Identifiers

PMID42140199
PMCPMC13261949

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.