Amelioration of Listeria monocytogenes infection in mice by live and pasteurized Faecalibacterium prausnitzii and its cell-free supernatant and its association with strengthened gut barrier and downregulated TLR4/NF-κB pathway.

Ailin Wang, Yue Teng, Jinhui Jia, Yunqi Gu, Jian Guo, Xiaodong Xia

Abstract read

In one paragraph

Article in Probiotics and antimicrobial proteins, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

6 authors.

Ailin WangState Key Laboratory of Marine Food Processing and Safety Control, National Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian, Liaoning, 116034, China.

Yue TengState Key Laboratory of Marine Food Processing and Safety Control, National Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian, Liaoning, 116034, China.

Jinhui JiaState Key Laboratory of Marine Food Processing and Safety Control, National Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian, Liaoning, 116034, China.

Yunqi GuState Key Laboratory of Marine Food Processing and Safety Control, National Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian, Liaoning, 116034, China.

Jian GuoState Key Laboratory of Marine Food Processing and Safety Control, National Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian, Liaoning, 116034, China.

Xiaodong XiaState Key Laboratory of Marine Food Processing and Safety Control, National Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian, Liaoning, 116034, China. xiaodong.xia@polyu.edu.hk.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Listeria monocytogenes is a common foodborne pathogen that poses a serious health risk. Faecalibacterium prausnitzii is one of the major members of the gut microbiota and is considered essential for maintaining gut health. The aim of this study was to investigate the effect of live F. prausnitzii (FP), pasteurized F. prausnitzii (pFP) and its cell-free supernatant (CFS) on the susceptibility of mice to L. monocytogenes infection and to explore the underlying mechanisms. Safety evaluation results indicated that FP, pFP, and CFS showed no toxic effects in healthy mice. In L. monocytogenes-infected mice, these treatments significantly decreased bacterial counts in various organs and feces, and alleviated inflammation. Furthermore, the interventions significantly upregulated short-chain fatty acid (SCFA) levels, particularly acetate and propionate, and modulated the gut microbiota composition after infection. In addition, RT-qPCR and western blot results showed that they reduced inflammatory factors and intestinal damage, which is associated with downregulation of the TLR4/NF-κB pathway. Taken together, these results suggest that FP, pFP and CFS attenuate L. monocytogenes infection in mice and could be potentially developed as an alternate strategy for prevention or mitigation of L. monocytogenes.

Indexed as

Faecalibacterium prausnitziiListeria monocytogenesListeriosisNF-kappa BToll-Like Receptor 4AnimalsDown-RegulationFemaleGastrointestinal MicrobiomeIntestinal Barrier FunctionMaleMicePasteurizationSignal TransductionNF-kappa BTlr4 protein, mouseToll-Like Receptor 4Faecalibacterium prausnitziiInfectionInflammatory responsesIntestinal barrier functionListeria monocytogenes

Identifiers

PMID42142241

DOI10.1007/s12602-026-11041-7

PMCPMC13518498

What Socratic holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.