Evidence map›Paper›PMID 42145249›Full record

ArticleMicrobiologyOpen2026

Exploring the Antimicrobial Potential of Vanadium-Based MXenes for Biomedical Applications.

Roberto Rosato, Andreas Rosenkranz, Giordano Perini, Giulia Santarelli, Alberto Augello, Dario F Zambrano, Iasi Cervantes, Nuria Abigail Plebani, Fernando Pablo Cometto, Marco De Spirito and 4 more

Abstract read
In one paragraph

Article in MicrobiologyOpen, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Roberto RosatoDepartment of Basic Biotechnological Sciences, Intensive and Perioperative Clinics, Università Cattolica del Sacro Cuore, Rome, Italy.ORCID 0000-0003-1570-6973
Andreas RosenkranzDepartment of Chemical Engineering, Biotechnology and Materials (FCFM), Universidad de Chile, Santiago, Chile.
Giordano PeriniDepartamento de Fisicoquímica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba, Argentina.
Giulia SantarelliDepartment of Basic Biotechnological Sciences, Intensive and Perioperative Clinics, Università Cattolica del Sacro Cuore, Rome, Italy.
Alberto AugelloDepartment of Neuroscience, Catholic University of the Sacred Heart, Rome, Italy.
Dario F ZambranoDepartment of Chemical Engineering, Biotechnology and Materials (FCFM), Universidad de Chile, Santiago, Chile.
Iasi CervantesDepartment of Chemical Engineering, Biotechnology and Materials (FCFM), Universidad de Chile, Santiago, Chile.
Nuria Abigail PlebaniDepartamento de Fisicoquímica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba, Argentina.
Fernando Pablo ComettoDepartamento de Fisicoquímica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba, Argentina.
Marco De SpiritoDepartment of Neuroscience, Catholic University of the Sacred Heart, Rome, Italy.
Maurizio SanguinettiDepartment of Basic Biotechnological Sciences, Intensive and Perioperative Clinics, Università Cattolica del Sacro Cuore, Rome, Italy.ORCID 0000-0002-9780-7059
Valentina PalmieriInstitute for Complex Systems, National Research Council (ISC-CNR), Rome, Italy.ORCID 0000-0002-6358-9647
Massimiliano PapiDepartment of Neuroscience, Catholic University of the Sacred Heart, Rome, Italy.ORCID 0000-0002-0029-1309
Flavio De MaioDepartment of Laboratory and Hematological Sciences, Fondazione Policlinico Universitario A. Gemelli IRCCS, Rome, Italy.ORCID 0000-0002-4744-9820

Funding

European Commission INF-ACTEuropean Commission PE00000007
6 · The paper itself

Abstract

MXenes, a family of two-dimensional transition metal carbides, carbonitrides and nitrides, have emerged as highly interesting antimicrobial nanomaterials. While mostly Ti-based MXenes have been explored in this field, our work aims at characterizing and investigating the antibacterial and biocompatibility profiles of two vanadium-based MXenes (V₂CTₓ and V₄C₃Tₓ) against Escherichia coli and Staphylococcus aureus, two clinically relevant pathobionts. First, the as-synthesized nanomaterials were chemically and structurally characterized to confirm their morphology and structural integrity. After setting up two distinct experimental models (static and dynamic), the antibacterial activity was evaluated by colony-forming units (CFUs) counting and scansion electron microscopy (SEM). Cellular cytotoxicity was assessed by lactate dehydrogenase (LDH) release and crystal violet characterization (CV). To further evaluate the MXenes' properties, the antimicrobial activity was tested in in-vitro infection models using both epithelial (Caco-2) and macrophage (J774) cells measuring CFUs. To assess the oxidative stress contributing to MXenes' antibacterial activity, reactive oxygen species (ROS) production was valued in infected cells after treatment. V₂CTₓ and V₄C₃Tₓ showed an antibacterial activity concentration and condition dependent. The dynamic incubation improved the bacterial reduction, supporting a "nano-knife" mechanism linked to the physical disruption of the membrane. Finally, V₂CTₓ and V₄C₃Tₓ significantly reduced the intracellular bacterial burden in infected Caco-2 epithelial cells in comparison with macrophages. Importantly, MXenes' treatment did not result in marked ROS stimulation, suggesting that their antibacterial activity mainly arose from physical interactions. Our findings highlight that vanadium-based MXenes have good biocompatibility and are moderately effective antimicrobial nanomaterials, emphasizing the need to use commonly recognized and standardized experimental models to elucidate their potential antimicrobial applications.

Indexed as

Anti-Bacterial AgentsAnti-Infective AgentsEscherichia coliNanostructuresStaphylococcus aureusVanadiumAnimalsCaco-2 CellsCell LineCell SurvivalColony Count, MicrobialHumansMacrophagesMiceMicrobial Sensitivity TestsReactive Oxygen SpeciesAnti-Bacterial AgentsAnti-Infective AgentsReactive Oxygen SpeciesVanadium2D materialsantimicrobial activityEscherichia coliinfection modelsMXenesStaphylococcus aureus

Identifiers

PMID42145249
PMCPMC13181600

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.