ReviewFrontiers in cellular neuroscience2026
Live-cell physiology in human brain tissue culture-the potential, the challenges, and the lessons learned.
Review in Frontiers in cellular neuroscience, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Adaptive Network Stabilization Despite Profound Structural Reorganization in Human Organotypic Cultures.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A growing number of studies are now utilizing human organotypic brain slice cultures (OBSC), enabled by improved long-term culture protocols that support the investigation of human-specific cellular physiology. Human OBSC offer the unique advantage, that they preserve the connectivity and microenvironment of human brain tissue while remaining experimentally tractable for live functional assays. However, methods established in rodent brain slice cultures, such as viral labeling, genetic manipulation, and long-term transduction strategies, do not readily translate to human tissue due to differences in viral tropism, tissue heterogeneity, and limited survival in culture. In this review, we highlight key historical developments and provide an overview of current best practices of human brain tissue culture. We provide an overview of live cell-specific labeling strategies, including viral approaches, as well as current and emerging technologies for functional manipulation and for investigating disease-related pathologies. Throughout, we integrate insights gained from mouse models and discuss how these technologies could potentially be adapted and leveraged for human OBSCs. Beyond methodological approaches, we also address the challenges and limitations inherent to working with human brain tissue. Together, these advances position human OBSCs as a powerful platform to bridge the gap between reductionist
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.