Evidence map›Paper›PMID 42147455›Full record

ArticleTransboundary and emerging diseases2026

Establishment of a Tetra-Primer ARMS-PCR Assay to Discriminate Genotypes I and II African Swine Fever Viruses.

Leilei Ding, Kun Xue, Tao Ren, Sihan Zhang, Weldu Tesfagaber, Zhenjiang Zhang, Feihu Guan, Jie Zhang, Yuanmao Zhu, Renqiang Liu and 5 more

Abstract read
In one paragraph

Article in Transboundary and emerging diseases, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Leilei DingState Key Laboratory of Animal Disease Control and Prevention, Professional Laboratory for African Swine Fever (Harbin), Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150069, China, caas.cn.ORCID https://orcid.org/0009-0006-7672-1551
Kun XueState Key Laboratory of Animal Disease Control and Prevention, Professional Laboratory for African Swine Fever (Harbin), Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150069, China, caas.cn.ORCID https://orcid.org/0009-0000-3198-7176
Tao RenCollege of Veterinary Medicine, Xinjiang Agricultural University, Urumqi, 830052, China, xjau.edu.cn.ORCID https://orcid.org/0009-0003-5056-957X
Sihan ZhangState Key Laboratory of Animal Disease Control and Prevention, Professional Laboratory for African Swine Fever (Harbin), Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150069, China, caas.cn.ORCID https://orcid.org/0009-0009-9270-3101
Weldu TesfagaberState Key Laboratory of Animal Disease Control and Prevention, Professional Laboratory for African Swine Fever (Harbin), Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150069, China, caas.cn.ORCID https://orcid.org/0000-0002-0695-2171
Zhenjiang ZhangState Key Laboratory of Animal Disease Control and Prevention, Professional Laboratory for African Swine Fever (Harbin), Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150069, China, caas.cn.ORCID https://orcid.org/0009-0000-4948-4227
Feihu GuanSHIHEZI University, Shihezi, 832000, Xinjiang Uygur Autonomous Region, China, shzu.edu.cn.ORCID https://orcid.org/0009-0000-9972-3863
Jie ZhangSHIHEZI University, Shihezi, 832000, Xinjiang Uygur Autonomous Region, China, shzu.edu.cn.ORCID https://orcid.org/0009-0004-5197-1816
Yuanmao ZhuState Key Laboratory of Animal Disease Control and Prevention, Professional Laboratory for African Swine Fever (Harbin), Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150069, China, caas.cn.ORCID https://orcid.org/0000-0002-7515-0324
Renqiang LiuState Key Laboratory of Animal Disease Control and Prevention, Professional Laboratory for African Swine Fever (Harbin), Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150069, China, caas.cn.ORCID https://orcid.org/0000-0001-6456-812X
Fang LiState Key Laboratory of Animal Disease Control and Prevention, Professional Laboratory for African Swine Fever (Harbin), Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150069, China, caas.cn.ORCID https://orcid.org/0009-0009-8190-0177
Zhigao BuState Key Laboratory of Animal Disease Control and Prevention, Professional Laboratory for African Swine Fever (Harbin), Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150069, China, caas.cn.ORCID https://orcid.org/0000-0001-9242-4211
Qianyi ZhangSHIHEZI University, Shihezi, 832000, Xinjiang Uygur Autonomous Region, China, shzu.edu.cn.ORCID https://orcid.org/0009-0004-1200-9368
Encheng SunState Key Laboratory of Animal Disease Control and Prevention, Professional Laboratory for African Swine Fever (Harbin), Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150069, China, caas.cn.ORCID https://orcid.org/0000-0002-4750-2329
Dongming ZhaoState Key Laboratory of Animal Disease Control and Prevention, Professional Laboratory for African Swine Fever (Harbin), Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150069, China, caas.cn.ORCID https://orcid.org/0000-0001-7161-6214

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The African swine fever virus (ASFV), particularly the genotypes I and II strains that have spread beyond Africa, continues to threaten global swine production and food security. Currently, African swine fever (ASF) control strategies rely heavily on biosecurity measures underpinned with molecular diagnostics. To address the need for genotyping, the present study developed a tetra-primer amplification refractory mutation system PCR (ARMS-PCR) assay capable of discriminating genotypes I and II ASFVs, as well as their mixed infections. The assay utilizes a multiplex of four primers: two outer universal primers and two allele-specific inner primers targeting a conserved single nucleotide polymorphism (SNP, G1656A) of the

Indexed as

African Swine FeverAfrican Swine Fever VirusPolymerase Chain ReactionAnimalsDNA PrimersGenotypePolymorphism, Single NucleotideSensitivity and SpecificitySwineDNA PrimersASFVdifferential detectiongenotype I and IISNPtetra-primer ARMS-PCR

Identifiers

PMID42147455
PMCPMC13176783

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.