A Protocol to Prepare and Stain Dorsal Skin Whole Mounts from Mice.

Natalia A Veniaminova, Marina Grachtchouk, Andrzej A Dlugosz, Sunny Y Wong

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Natalia A VeniaminovaDepartment of Dermatology, Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, MI, USA.

Marina GrachtchoukDepartment of Dermatology, Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, MI, USA.

Andrzej A DlugoszDepartment of Dermatology, Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, MI, USA.

Sunny Y WongDepartment of Dermatology, Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, MI, USA. sunnyw@umich.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Tissue whole mounts have long been used to assess morphological changes following genetic perturbation in mice. Traditional methods for preparing dorsal skin whole mounts, however, often lead to breakage of hair follicle structures, such as sebaceous glands, making it difficult to perform unbiased quantitation. Here, we describe a modified technique for preparing whole mounts harvested from mouse dorsal skin. This protocol incorporates adhesives to stiffen the tissue and facilitate the separation of intact epidermis from the dermis. Critically, this method is compatible with downstream applications such as lipid staining of sebaceous glands and immunofluorescence, and we provide protocols for both approaches. Overall, the use of this modified "glue technique" for preparing skin whole mounts will help preserve tissue integrity for morphological analysis.

Indexed as

SkinStaining and LabelingAnimalsDermisEpidermisFluorescent Antibody TechniqueHair FollicleMiceSebaceous GlandsEpidermisHair folliclePilosebaceous unitPPARGSCD1Sebaceous gland

Identifiers

PMID42149447

DOI10.1016/j.devcel.2019.08.015

What Socratic holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.