ArticleCurrent protocols2026
Identifying Donor Immune Cells in the Intestinal Mucosa of Chimeric Recipient Mice by Immunofluorescence.
Article in Current protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Identifying Donor Immune Cells in the Intestinal Mucosa of Chimeric Recipient Mice by Immunofluorescence.Current protocols · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
Murine competitive congenic bone marrow transplantation is an important method used to identify genetic contributions to immune cell abundance, activation, differentiation, and mobilization, either in the bone marrow or in the peripheral organs of the host. This enables analysis of interactions between cells from both donors in relation to each other and the host itself. Flow cytometry is the predominant approach for experimental designs such as these for its powerful quantitative capacity and ability to identify rare and complex immune cells using cell surface markers. However, information regarding immune cell localization and interaction in peripheral organs is usually unexplored due to technical difficulties in identifying immune cells through immunofluorescence, as tissue fixation results in protein cross-linking and inadvertent masking of protein epitopes. In addition, commercially available antibodies validated for flow cytometry are generally designed to interact with integral epitopes expressed on the outer cell membrane. This feature renders conventional immunofluorescence using formalin-fixed tissues impractical because antigen retrieval techniques result in breakage of cross-linked proteins by the denaturation process. Here, we have adapted and improved routinely used immunofluorescence protocols to enable identification of CD45
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.