Evidence map›Paper›PMID 42150419›Full record

ArticlePoultry science2026

Age-associated miR-30e-3p upregulation drives ovarian follicular granulosa cell senescence by targeting DPY30 in laying hens.

Yuanhang Wei, Yixuan Wang, Chimei Liao, Can Cui, Yao Zhang, Zhuanjian Li, Congjiao Sun, Qing Zhu, Huadong Yin

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Poultry science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Yuanhang WeiState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, Sichuan, China; Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, Sichuan, China.
Yixuan WangState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, Sichuan, China; Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, Sichuan, China.
Chimei LiaoState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, Sichuan, China; Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, Sichuan, China.
Can CuiState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, Sichuan, China; Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, Sichuan, China.
Yao ZhangState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, Sichuan, China; Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, Sichuan, China.
Zhuanjian LiCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450002, Henan, China.
Congjiao SunDepartment of Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
Qing ZhuState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, Sichuan, China; Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, Sichuan, China.
Huadong YinState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, Sichuan, China; Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, Sichuan, China. Electronic address: yinhuadong@sicau.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Ovarian aging in laying hens contributes significantly to the decline in egg production performance during the late laying period, and is accompanied by extensive remodeling of miRNA-mRNA interaction networks, which are critical post-transcriptional regulators during this process. In this study, we investigated the role of miR-30e-3p, which was previously identified as significantly upregulated during ovarian aging in laying hens, in granulosa cell (GC) senescence. To identify its downstream effectors, we performed mRNA transcriptome sequencing and screened for downregulated genes. Among potential targets, the histone methyltransferase complex regulatory subunit dpy-30 (DPY30) was validated as a direct target of miR-30e-3p by luciferase reporter and expression analyses. Functional assays demonstrated that miR-30e-3p significantly suppressed GC proliferation and induced cell cycle arrest, whereas DPY30 exhibited opposite effects. Furthermore, inhibition of miR-30e-3p or overexpression of DPY30 attenuated D-galactose (D-gal) -induced GC senescence. Mechanistically, miR-30e-3p promoted cellular senescence at least in part through downregulation of DPY30. Collectively, our findings indicated that miR-30e-3p promotes GC senescence by targeting DPY30 in chicken ovarian follicles, and suggest that both miR-30e-3p and DPY30 may serve as potential biomarkers for improving reproductive longevity and mitigating ovarian aging-related disorders in poultry.

Indexed as

Avian ProteinsCellular SenescenceChickensGranulosa CellsHistone MethyltransferasesMicroRNAsAgingAnimalsFemaleOvarian FollicleUp-RegulationAvian ProteinsHistone MethyltransferasesMicroRNAsCellular senescenceDPY30miR-30e-3pOvarian aging

Identifiers

PMID42150419
PMCPMC13208791

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.