Evidence map›Paper›PMID 42154595›Full record

ArticleSTAR protocols2026

Protocol for recombinant expression in E. coli and purification of iBoost vaccine proteins using immobilized metal affinity chromatography.

Elisabeth J M Huijbers, Judy R van Beijnum, Joost Koetsier, Gabriela E Wachholz, Peter C J Kemper, Lauren Myburgh, Steijn Meister, Karlijn van Loon, Mattie A Cassee, Arjan W Griffioen

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Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Elisabeth J M HuijbersAngiogenesis Laboratory, Department of Medical Oncology, Amsterdam UMC, Cancer Center Amsterdam, 1081 HV Amsterdam, the Netherlands; CimCure BV, 1066 CX Amsterdam, the Netherlands. Electronic address: e.huijbers@amsterdamumc.nl.
Judy R van BeijnumAngiogenesis Laboratory, Department of Medical Oncology, Amsterdam UMC, Cancer Center Amsterdam, 1081 HV Amsterdam, the Netherlands.
Joost KoetsierAngiogenesis Laboratory, Department of Medical Oncology, Amsterdam UMC, Cancer Center Amsterdam, 1081 HV Amsterdam, the Netherlands.
Gabriela E WachholzAngiogenesis Laboratory, Department of Medical Oncology, Amsterdam UMC, Cancer Center Amsterdam, 1081 HV Amsterdam, the Netherlands; CimCure BV, 1066 CX Amsterdam, the Netherlands.
Peter C J KemperAngiogenesis Laboratory, Department of Medical Oncology, Amsterdam UMC, Cancer Center Amsterdam, 1081 HV Amsterdam, the Netherlands.
Lauren MyburghAngiogenesis Laboratory, Department of Medical Oncology, Amsterdam UMC, Cancer Center Amsterdam, 1081 HV Amsterdam, the Netherlands.
Steijn MeisterAngiogenesis Laboratory, Department of Medical Oncology, Amsterdam UMC, Cancer Center Amsterdam, 1081 HV Amsterdam, the Netherlands.
Karlijn van LoonAngiogenesis Laboratory, Department of Medical Oncology, Amsterdam UMC, Cancer Center Amsterdam, 1081 HV Amsterdam, the Netherlands.
Mattie A CasseeAngiogenesis Laboratory, Department of Medical Oncology, Amsterdam UMC, Cancer Center Amsterdam, 1081 HV Amsterdam, the Netherlands.
Arjan W GriffioenAngiogenesis Laboratory, Department of Medical Oncology, Amsterdam UMC, Cancer Center Amsterdam, 1081 HV Amsterdam, the Netherlands; CimCure BV, 1066 CX Amsterdam, the Netherlands. Electronic address: a.griffioen@amsterdamumc.nl.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The iBoost vaccine technology enables the induction of a humoral immune response against self- and low-immunogenic antigens through fusion of the antigen to an immunogenic bacterial carrier. Here, we present a protocol for recombinant expression in E. coli and purification of iBoost vaccine proteins using immobilized metal affinity chromatography (IMAC). In addition, we describe procedures to evaluate antigen-specific immune responses in mice. This protocol can be readily adapted for the generation and testing of vaccines against a broad range of antigens. For complete details on the use and execution of this protocol, please refer to Huijbers et al., Van Beijnum et al., and Van Loon et al.

Indexed as

Chromatography, AffinityEscherichia coliRecombinant ProteinsAnimalsMetalsMiceMetalsRecombinant ProteinsBiotechnology and bioengineeringCancerCell BiologyImmunologyMolecular BiologyProtein BiochemistryProtein expression and purification

Identifiers

PMID42154595
PMCPMC13213800

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.