Evidence map›Paper›PMID 42157296›Full record

ArticleBiotechnology for biofuels and bioproducts2026

Direct valorization of agricultural byproducts to vanillin by a synergistic Komagataella phaffii co-culture system.

Difei Zhou, Yuji Yang, Yi Shao, Lina Sun, Geqi Qi, Menghui Tao, Zhen Li, Fengjiao Xin

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biotechnology for biofuels and bioproducts, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Difei Zhou *Laboratory of Biomanufacturing and Food Engineering, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
Yuji Yang *Laboratory of Biomanufacturing and Food Engineering, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
Yi ShaoLaboratory of Biomanufacturing and Food Engineering, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
Lina SunLaboratory of Biomanufacturing and Food Engineering, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
Geqi QiInstitute of Agricultural and Animal Husbandry of Hinggan League, Inner Mongolia Innovation Center of Biological Breeding Technology, Inner Mongolia Key Laboratory of Rice Breeding Innovation in Northern Cold Regions, Ulanhot, 137400, Inner Mongolia, China.
Menghui TaoInstitute of Agricultural and Animal Husbandry of Hinggan League, Inner Mongolia Innovation Center of Biological Breeding Technology, Inner Mongolia Key Laboratory of Rice Breeding Innovation in Northern Cold Regions, Ulanhot, 137400, Inner Mongolia, China.
Zhen LiLaboratory of Biomanufacturing and Food Engineering, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, 100193, China. lizhen19890911@163.com.
Fengjiao XinLaboratory of Biomanufacturing and Food Engineering, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, 100193, China. 2002hongzhi30@163.com.

Funding

the Beijing Natural Science Foundation No.5232023the Central Government Guiding Local Science and Technology Development Funds of Hebei Province No.236Z2802Gthe financial support from the Central Public-interest Scientific Institution Basal Research Fund No. Y2024QC13the National Natural Science Foundation of China No.52300186the Xing'an League Local-Level Science and Technology Targeted Cooperation Project MBDHZ2024007
6 · The paper itself

Abstract

backgroundThe annual abundance of lignocellulosic agricultural byproducts poses a significant environmental burden. Leveraging this underutilized biomass to produce high-value, food-grade products represents a promising strategy for diversifying food ingredient sources and advancing the principles of the circular bioeconomy.

resultsHere, a novel Komagataella phaffii co-culture system was developed for integrated xylan hydrolysis and vanillin biosynthesis. In the upstream xylan hydrolysis module, overexpression of a novel multi-modular bifunctional xylanase/feruloyl esterase enabled the direct hydrolysis of xylans in agricultural byproducts to release ferulic acid (FA), thereby providing precursors for the downstream vanillin synthesis module. In the downstream module, FA was converted to vanillin via heterologous expression of feruloyl-CoA synthetase (FCS) and enoyl-CoA hydratase/aldolase (ECH). Subsequent optimization of strain inoculation ratios and carbon source composition in the co-culture system increased the titer of vanillin and its derivatives to 2.63 mM, achieving a 47.90% molar conversion rate from ultrafine-grinding corn cob (UGCC).

conclusionsThis study establishes a scalable platform for synthesizing aromatic compounds from low-cost lignocellulosic feedstocks, offering a strategic blueprint for valorizing agricultural residues.

Indexed as

Agricultural byproductsCo-cultureKomagataella phaffiiVanillin biosynthesisXylan hydrolysis

Identifiers

PMID42157296
PMCPMC13352825

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.