Evidence map›Paper›PMID 42166333›Full record

ArticleSTAR protocols2026

Protocol for determining minimum inhibitory concentrations of essential oils against bacterial pathogens using broth microdilution.

Maura Di Vito, Melinda Mariotti, Mattia Di Mercurio, Domiziana Coggiatti, Silvia Rizzo, Abdesselam Zhiri, Francesca Bugli, Maurizio Sanguinetti, Brunella Posteraro

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Maura Di VitoDipartimento di Scienze Biotecnologiche di Base, Cliniche Intensivologiche e Perioperatorie, Università Cattolica del Sacro Cuore, Rome, Italy. Electronic address: maura.divito@unicatt.it.
Melinda MariottiDipartimento di Scienze Biotecnologiche di Base, Cliniche Intensivologiche e Perioperatorie, Università Cattolica del Sacro Cuore, Rome, Italy.
Mattia Di MercurioDipartimento di Scienze Biotecnologiche di Base, Cliniche Intensivologiche e Perioperatorie, Università Cattolica del Sacro Cuore, Rome, Italy.
Domiziana CoggiattiDipartimento di Scienze Biotecnologiche di Base, Cliniche Intensivologiche e Perioperatorie, Università Cattolica del Sacro Cuore, Rome, Italy.
Silvia RizzoDipartimento di Scienze Biotecnologiche di Base, Cliniche Intensivologiche e Perioperatorie, Università Cattolica del Sacro Cuore, Rome, Italy.
Abdesselam ZhiriR&D Department, Pranarôm International S.A., Ghislenghien, Belgium; Plant Biotechnology Research Unit, Université Libre de Bruxelles, Brussels, Belgium.
Francesca BugliDipartimento di Scienze Biotecnologiche di Base, Cliniche Intensivologiche e Perioperatorie, Università Cattolica del Sacro Cuore, Rome, Italy; Dipartimento di Scienze di Laboratorio ed Ematologiche, Fondazione Policlinico Universitario a. Gemelli IRCCS, Rome, Italy.
Maurizio SanguinettiDipartimento di Scienze Biotecnologiche di Base, Cliniche Intensivologiche e Perioperatorie, Università Cattolica del Sacro Cuore, Rome, Italy; Dipartimento di Scienze di Laboratorio ed Ematologiche, Fondazione Policlinico Universitario a. Gemelli IRCCS, Rome, Italy. Electronic address: maurizio.sanguinetti@unicatt.it.
Brunella PosteraroDipartimento di Scienze Biotecnologiche di Base, Cliniche Intensivologiche e Perioperatorie, Università Cattolica del Sacro Cuore, Rome, Italy; Unità Operativa "Medicina di Precisione in Microbiologia Clinica", Direzione Scientifica, Fondazione Policlinico Universitario a. Gemelli IRCCS, Rome, Italy.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Essential oils (EOs) are widely investigated for antimicrobial activity, yet minimum inhibitory concentrations (MICs) lack cross-study comparability due to the absence of a harmonized reference assay. Here, we present a protocol to determine MICs of EOs against aerobic bacterial pathogens using a broth microdilution technique. We describe steps for incorporating EO dispersion using Tween 80 and preparing a standardized bacterial inoculum. We detail procedures for establishing two-fold dilution series in 96-well plates and visually determining the MIC endpoint.

Indexed as

Anti-Bacterial AgentsBacteriaOils, VolatileMicrobial Sensitivity TestsAnti-Bacterial AgentsOils, VolatileChemistryHealth SciencesMicrobiology

Identifiers

PMID42166333
PMCPMC13199665

What Socratic holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.