Evidence map›Paper›PMID 42168713›Full record

ArticleJournal of assisted reproduction and genetics2026

A multidimensional follicular profile integrating oxidative stress, apoptosis and PI3K/AKT/mTOR signaling in relation to oocyte developmental competence in in vitro fertilization cycles.

Oya Korkmaz, Seda Karabulut, Pelin Macit, İlknur Keskin

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Article in Journal of assisted reproduction and genetics, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Oya KorkmazDepartment of Histology and Embryology, Faculty of Medicine, Malatya Turgut Özal University, Malatya, Turkey. korkmazoya@yahoo.com.
Seda KarabulutDepartment of Histology and Embryology, International Faculty of Medicine, Istanbul Medipol University, Istanbul, Turkey. sedakarabulut@medipol.edu.tr.
Pelin MacitIn Vitro Fertilization Center, Istanbul Okan University Hospital, Istanbul, Turkey.
İlknur KeskinDepartment of Histology and Embryology, Faculty of Medicine, Istanbul Medipol University, Istanbul, Turkey.

Funding

İstanbul Medipol University Scientific Research Projects Commission Project No: 2020/18
6 · The paper itself

Abstract

purposeAccurate assessment of oocyte developmental competence remains challenging in IVF, as morphology alone offers limited insight into intrinsic oocyte quality. Emerging evidence indicates that oocyte competence reflects coordinated regulation of the follicular microenvironment rather than a single biomarker. This study aimed to evaluate a multidimensional follicular marker panel integrating oxidative stress parameters, hormonal environment, granulosa cell apoptosis, chromatin integrity and PTEN/PI3K/AKT/mTOR signaling in relation to IVF outcomes.

methodsThirty women undergoing ICSI were included, yielding a total of 211 oocytes. Individual follicles were analyzed according to oocyte maturity, embryo quality and pregnancy outcome. Follicular fluid oxidative stress parameters (TAC, TOC, OSI and MDA) and hormonal mediators (FSH, AMH, melatonin, TGF-β and β-hCG) were assessed. Granulosa cells were evaluated for DNA fragmentation, chromatin integrity and expression of PTEN/PI3K/AKT/mTOR pathway proteins.

resultsFavorable IVF outcomes were associated with higher total antioxidant capacity and lower oxidative stress index and malondialdehyde levels, alongside reduced granulosa cell DNA fragmentation and improved chromatin integrity (p < 0.05). These outcomes were characterized by increased PI3K and AKT expression with concomitant mTOR suppression, while PTEN, PDK1, TSC1 and TSC2 levels remained unchanged. Follicular FSH and AMH were primarily linked to oocyte maturity, whereas melatonin, TGF-β and β-hCG were elevated in follicles yielding good-quality embryos and positive pregnancy outcomes (p < 0.05).

conclusionsOocyte developmental competence in IVF reflects a coordinated follicular microenvironment integrating oxidative balance, granulosa cell integrity, hormonal regulation, and PI3K/AKT/mTOR signaling, supporting a multidimensional, panel-based framework for integrated follicular assessment.

Indexed as

ApoptosisFollicular fluidOocytesOxidative stressPI3K/AKT/mTOR signaling

Identifiers

PMID42168713

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.