Evidence map›Paper›PMID 42170385›Full record

ArticleBiochemistry and biophysics reports2026

Long-read transcriptomic profiling of a glioma cell line following m6A regulator perturbations.

Hanna Lee, Syeda Maheen Batool, Ana K Escobedo, Denalda Gashi, Kesli Faber, Pavithra Ponnolu, Nina R Barretts, Prerna Khanna, Kase D Haas, Tiffaney Hsia and 2 more

Abstract read
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In one paragraph

Article in Biochemistry and biophysics reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Hanna LeeDepartment of Neurosurgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
Syeda Maheen BatoolDepartment of Neurosurgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
Ana K EscobedoDepartment of Neurosurgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
Denalda GashiDepartment of Neurosurgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
Kesli FaberDepartment of Neurosurgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
Pavithra PonnoluDepartment of Neurosurgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
Nina R BarrettsDepartment of Neurosurgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
Prerna KhannaDepartment of Neurosurgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
Kase D HaasDepartment of Neurosurgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
Tiffaney HsiaDepartment of Neurosurgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
Bob S CarterDepartment of Neurosurgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
Leonora BalajDepartment of Neurosurgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

N6-methyladenosine (m6A) is the most prevalent internal mRNA modification and is enriched in the central nervous system (CNS), yet its role in glioma remains incompletely defined. Using long-read direct RNA sequencing, we mapped transcriptome-wide m6A modifications in a single glioma cell line following targeted knockdown of the m6A reader IGF2BP2, writer METTL3, and eraser ALKBH5. Across perturbations, the global architecture of m6A, including transcript class, positional enrichment, and site multiplicity, was largely preserved, while differential methylation was weakly coupled to gene expression. In contrast, m6A regulator perturbation coincided with widespread isoform switching and with changes in untranslated regions, coding potential, and predicted transcript fate, largely independent of bulk gene expression changes. Public glioma datasets further supported the relevance of isoform-specific changes in glioma. Together, these findings highlight isoform-level transcript variation associated with m6A regulator perturbation and support the use of long-read, isoform-resolved approaches to study RNA regulatory states in glioma.

Indexed as

Alternative splicingEpitranscriptomicsGliomaIsoformN6-methyladenosine (m6A)RNASignaling

Identifiers

PMID42170385
PMCPMC13187546

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.