Evidence map›Paper›PMID 42176253›Full record

ArticleBiology of reproduction2026

A feeder-free culture system supports long-term expansion and germline competence of bovine formative embryonic stem cells†.

Lei Chen, Furui Wang, Jiale He, Mingwei Sun, Yiru Wang, Yinjuan Wang, Shumin Wang, Lei An, Guangyin Xi, Jianhui Tian

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biology of reproduction, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Lei ChenFrontiers Science Center for Molecular Design Breeding (MOE), State Key Laboratory of Animal Biotech Breeding, Key Laboratory of Animal Genetics, Breeding and Reproduction (Livestock), Ministry of Agriculture and Rural Affairs, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Haidian, Beijing, 100193, China.ORCID 0000-0002-5400-968X
Furui WangFrontiers Science Center for Molecular Design Breeding (MOE), State Key Laboratory of Animal Biotech Breeding, Key Laboratory of Animal Genetics, Breeding and Reproduction (Livestock), Ministry of Agriculture and Rural Affairs, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Haidian, Beijing, 100193, China.
Jiale HeFrontiers Science Center for Molecular Design Breeding (MOE), State Key Laboratory of Animal Biotech Breeding, Key Laboratory of Animal Genetics, Breeding and Reproduction (Livestock), Ministry of Agriculture and Rural Affairs, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Haidian, Beijing, 100193, China.
Mingwei SunFrontiers Science Center for Molecular Design Breeding (MOE), State Key Laboratory of Animal Biotech Breeding, Key Laboratory of Animal Genetics, Breeding and Reproduction (Livestock), Ministry of Agriculture and Rural Affairs, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Haidian, Beijing, 100193, China.
Yiru WangFrontiers Science Center for Molecular Design Breeding (MOE), State Key Laboratory of Animal Biotech Breeding, Key Laboratory of Animal Genetics, Breeding and Reproduction (Livestock), Ministry of Agriculture and Rural Affairs, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Haidian, Beijing, 100193, China.
Yinjuan WangFrontiers Science Center for Molecular Design Breeding (MOE), State Key Laboratory of Animal Biotech Breeding, Key Laboratory of Animal Genetics, Breeding and Reproduction (Livestock), Ministry of Agriculture and Rural Affairs, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Haidian, Beijing, 100193, China.
Shumin WangFrontiers Science Center for Molecular Design Breeding (MOE), State Key Laboratory of Animal Biotech Breeding, Key Laboratory of Animal Genetics, Breeding and Reproduction (Livestock), Ministry of Agriculture and Rural Affairs, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Haidian, Beijing, 100193, China.
Lei AnFrontiers Science Center for Molecular Design Breeding (MOE), State Key Laboratory of Animal Biotech Breeding, Key Laboratory of Animal Genetics, Breeding and Reproduction (Livestock), Ministry of Agriculture and Rural Affairs, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Haidian, Beijing, 100193, China.ORCID 0000-0001-8509-2466
Guangyin XiFrontiers Science Center for Molecular Design Breeding (MOE), State Key Laboratory of Animal Biotech Breeding, Key Laboratory of Animal Genetics, Breeding and Reproduction (Livestock), Ministry of Agriculture and Rural Affairs, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Haidian, Beijing, 100193, China.
Jianhui TianFrontiers Science Center for Molecular Design Breeding (MOE), State Key Laboratory of Animal Biotech Breeding, Key Laboratory of Animal Genetics, Breeding and Reproduction (Livestock), Ministry of Agriculture and Rural Affairs, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Haidian, Beijing, 100193, China.ORCID 0000-0003-4509-6336

Funding

China Agricultural University Basic Scientific Research Fund Program 2022TC114Major Science and Technology Project of Xinjiang Autonomous Region 2023A02011National Key Research and Development Program of China 2022YFD1302202
6 · The paper itself

Abstract

Bovine embryonic stem cells (bESCs) are a cornerstone for next-generation applications such as cell-cultured meat, large-animal gene editing, and embryo-stem cell breeding systems. Although primed bESCs and bovine expanded potential stem cells have recently been established, their routine maintenance commonly relies on mouse embryonic fibroblast or bovine fetal fibroblast feeder layers, which are complex, labor-intensive, poorly standardized, and incompatible with scalable manufacturing. Here, we report a simplified feeder-free culture platform for bESCs in which a commercially available basal medium is combined with Growth Factor Reduced Matrigel to generate a three-dimensional, niche-mimetic substrate. Using this approach, we successfully derived a novel feeder-free bESCs (FF-bESCs) that can be robustly expanded for more than 60 passages without ROCK inhibitors, while maintaining a normal karyotype, high proliferation rates, and stable expression of core pluripotency markers. Functional assays confirmed that FF-bESCs possess bona fide pluripotency, as evidenced by their ability to form embryoid bodies in vitro and generate teratomas containing derivatives of all three germ layers in vivo. Transcriptomic profiling further revealed that FF-bESCs align with a formative pluripotent state. Notably, these cells can be directed to differentiate into primordial germ cell-like cells. Our feeder-free culture system provides a scalable, reproducible foundation for advancing bESC-based technologies in bovine precision genome editing and germ cell differentiation for embryo-stem cell breeding systems.

Indexed as

Cell Culture TechniquesEmbryonic Stem CellsGerm CellsAnimalsCattleCell DifferentiationCell ProliferationCells, Culturedbovineembryonic stem cellsfeeder-freeformative pluripotencyprimordial germ cells

Identifiers

PMID42176253
PMCPMC13369327

What Socratic holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.