Evidence map›Paper›PMID 42178683›Full record

ArticleVox sanguinis2026

Factors associated with aggregate formation in cold-stored platelets.

Samantha Huang, Yuko Mishima, S Lawrence Bailey, Aastha Chauhan, José Quesada, Pat Klotz, Tyler Morrow, Kathryn Provencher, Junmei Chen, José A López and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Vox sanguinis, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Samantha HuangBloodworks Northwest Research Institute, Seattle, Washington, USA.
Yuko MishimaBloodworks Northwest Research Institute, Seattle, Washington, USA.
S Lawrence BaileyBloodworks Northwest Research Institute, Seattle, Washington, USA.
Aastha ChauhanBloodworks Northwest Research Institute, Seattle, Washington, USA.
José QuesadaDepartment of Manufacturing and Transfusion Services, South Texas Blood and Tissue Center, San Antonio, Texas, USA.
Pat KlotzBloodworks Northwest Research Institute, Seattle, Washington, USA.
Tyler MorrowDepartment of Blood Transfusion Service, Swedish Medical Center, Cherry Hill Campus, Seattle, Washington, USA.
Kathryn ProvencherDepartment of Blood Transfusion Service, Swedish Medical Center, Cherry Hill Campus, Seattle, Washington, USA.
Junmei ChenBloodworks Northwest Research Institute, Seattle, Washington, USA.ORCID https://orcid.org/0000-0002-6227-4088
José A LópezBloodworks Northwest Research Institute, Seattle, Washington, USA.
Tsung-Lin TsaiDepartment of Medical Services, Bloodworks Northwest, Seattle, Washington, USA.
Sandhya PanchDepartment of Medicine, Division of Hematology and Oncology, University of Washington, Seattle, Washington, USA.
Moritz StollaBloodworks Northwest Research Institute, Seattle, Washington, USA.ORCID https://orcid.org/0000-0002-2866-1674

Funding

U.S. Department of Defense EDMS 5570U.S. Department of Defense W81XWH-12-1-0441
6 · The paper itself

Abstract

BACKGROUND AND

objectivesCold-stored platelets (CSPs) can form aggregates, ultimately rendering them unusable. The causes of these aggregates and how to prevent them are poorly understood. This study aimed to identify potential factors associated with aggregate formation in CSPs stored in plasma for up to 14 days. MATERIALS AND

methodsWe obtained CSPs from 79 unique donors during a clinical trial. We retrospectively analysed aggregate rates among donors of different sexes, ages and blood groups. In a subgroup, samples were also tested by enzyme-linked immunosorbent assay for markers of thrombosis and inflammation. Platelets from mice lacking von Willebrand factor (VWF) and human apheresis platelets treated with VWF and caplacizumab were stored and assessed for platelet count and aggregate formation by visual inspection and flow cytometry.

resultsForty-five (57%) units developed aggregates, and 34 (43%) did not. Units with aggregates had significantly lower soluble thrombomodulin levels and higher VWF levels approaching significance. In a multivariate analysis, platelets from female donors and donors with ABO type A were significantly more likely to form aggregates than those from male donors and donors with ABO type O, respectively. In the oldest donor cohort, units with aggregates were significantly less common than those without. Interfering with VWF levels and VWF function during storage did not reliably cause or prevent aggregates.

conclusionDonor sex, age, ABO type and thrombomodulin were significantly associated with aggregate formation or prevention.

Indexed as

Blood PlateletsBlood PreservationCryopreservationPlatelet AggregationAdultAnimalsCold TemperatureFemaleHumansMaleMiceMiddle AgedRetrospective StudiesSex Factorsvon Willebrand Factorvon Willebrand Factorcardiothoracic surgerycold‐stored plateletsplatelet storageplatelet transfusion

Identifiers

PMID42178683
PMCPMC13453929

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.