Evidence map›Paper›PMID 42179739›Full record

ArticleSynthetic and systems biotechnology2026

Efficient whole-cell biocatalytic synthesis of 2'-deoxy-2'-fluoroadenosine, a key building block for nucleic acid drugs.

Dandan Feng, Tianshu Zhang, Guan Zhou, Yanli Cao, Quan Luo, Huifang Xu, Xuefeng Lu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Synthetic and systems biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Dandan FengKey Laboratory of Photoelectric Conversion and Utilization of Solar Energy, Shandong Provincial Center of Technology Innovation for Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling Road, Qingdao, 266101, China.
Tianshu ZhangKey Laboratory of Photoelectric Conversion and Utilization of Solar Energy, Shandong Provincial Center of Technology Innovation for Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling Road, Qingdao, 266101, China.
Guan ZhouKey Laboratory of Photoelectric Conversion and Utilization of Solar Energy, Shandong Provincial Center of Technology Innovation for Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling Road, Qingdao, 266101, China.
Yanli CaoKey Laboratory of Photoelectric Conversion and Utilization of Solar Energy, Shandong Provincial Center of Technology Innovation for Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling Road, Qingdao, 266101, China.
Quan LuoKey Laboratory of Photoelectric Conversion and Utilization of Solar Energy, Shandong Provincial Center of Technology Innovation for Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling Road, Qingdao, 266101, China.
Huifang XuKey Laboratory of Photoelectric Conversion and Utilization of Solar Energy, Shandong Provincial Center of Technology Innovation for Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling Road, Qingdao, 266101, China.
Xuefeng LuKey Laboratory of Photoelectric Conversion and Utilization of Solar Energy, Shandong Provincial Center of Technology Innovation for Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling Road, Qingdao, 266101, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

2'-Deoxy-2'-fluoroadenosine (2'-F-dA) is a nucleoside analogue used as a key building block for oligonucleotide drugs. It can be biosynthesized from a low-cost 2'-deoxy-2'-fluorouridine via one-pot transglycosylation catalyzed by a thymidine phosphorylase (TP) and a purine nucleoside phosphorylase (PNP). However, reliance on purified enzymes and low space-time yields present challenges for industrial application of the process. Here, we develop a whole-cell-based biocatalytic system employing TP and PNP from

Indexed as

2′-deoxy-2′-fluoroadenosineNucleoside analoguesNucleoside phosphorylasesThermal pretreatmentWhole-cell-based biosynthesis

Identifiers

PMID42179739
PMCPMC13198325

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.