Evidence mapPaperPMID 42185694Full record

ArticleNature cardiovascular research2026

Consensus statement on mass spectrometry-based proteomic analysis of cardiac tissue.

Alicia Lundby, Jennifer E Van Eyk, Manuel Mayr, Melanie Y White, Jonathan A Kirk, Jonathan S Achter, Justyna Fert-Bober, Michael Wierer, Philipp Mertins, Maggie P Y Lam and 5 more

Abstract readConsensus Statement
PubMed Publisher
In one paragraph

Article in Nature cardiovascular research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Alicia LundbyDepartment of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark. alicia.lundby@sund.ku.dk.ORCID http://orcid.org/0000-0002-1612-6041
Jennifer E Van EykSmidt Heart Institute, Cedars-Sinai Medical Center, Los Angeles, CA, USA.ORCID http://orcid.org/0000-0001-9050-148X
Manuel MayrNational Heart and Lung Institute, Imperial College London, London, UK.ORCID http://orcid.org/0000-0002-0597-829X
Melanie Y WhiteSchool of Medical Sciences, Faculty of Medicine and Health, University of Sydney, Sydney, New South Wales, Australia.ORCID http://orcid.org/0000-0003-2571-2816
Jonathan A KirkDepartment of Medicine, Section of Cardiology, University of Chicago, Chicago, IL, USA.
Jonathan S AchterDepartment of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.ORCID http://orcid.org/0000-0002-1587-622X
Justyna Fert-BoberSmidt Heart Institute, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
Michael WiererProteomics Research Infrastructure, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.ORCID http://orcid.org/0000-0002-0356-5571
Philipp MertinsMax Delbrück Center for Molecular Medicine, Berlin, Germany.ORCID http://orcid.org/0000-0002-2245-528X
Maggie P Y LamDepartment of Medicine, University of Colorado School of Medicine, Aurora, CO, USA.
Sean J HumphreyMurdoch Children's Research Institute, Royal Children's Hospital, Parkville, Victoria, Australia.ORCID http://orcid.org/0000-0002-2666-9744
Edward LauDepartment of Medicine, University of Colorado School of Medicine, Aurora, CO, USA.
Anthony O GramoliniDepartment of Physiology, University of Toronto, Toronto, Ontario, Canada.ORCID http://orcid.org/0000-0003-1109-2070
Ying GeDepartment of Cell and Regenerative Biology, Department of Chemistry, University of Wisconsin-Madison, Madison, WI, USA.ORCID http://orcid.org/0000-0001-5211-6812
Rebekah L GundryDepartment of Cellular and Integrative Physiology, and CardiOmics Program, Center for Heart and Vascular Research, University of Nebraska Medical Center, Omaha, NE, USA.ORCID http://orcid.org/0000-0002-9263-833X

Funding

Structural and functional basis of myocardial dysregulation in genetic cardiomyopathyR01HL172492 · LOYOLA UNIVERSITY CHICAGO · 2025 to 2025
$682k
Cardiac Sarcomere Protein Quality Control in Health and DiseaseR01HL136737 · LOYOLA UNIVERSITY CHICAGO · 2025 to 2025
$539k
Deutsche Forschungsgemeinschaft (German Research Foundation) 437531118-SFB-1470-B05Novo Nordisk Fonden (Novo Nordisk Foundation) NNF20OC0059767Novo Nordisk Fonden (Novo Nordisk Foundation) NNF25OC0097951U.S. Department of Health & Human Services | National Institutes of Health (NIH) 1R01HL155346-01A1U.S. Department of Health & Human Services | National Institutes of Health (NIH) 1U54CA260591-01U.S. Department of Health & Human Services | National Institutes of Health (NIH) R01HL136737U.S. Department of Health & Human Services | National Institutes of Health (NIH) R01HL172492U.S. Department of Health & Human Services | National Institutes of Health (NIH) R35-HL155460
6 · The paper itself

Abstract

Mass spectrometry-based cardiac proteomics provides direct molecular insight into cardiac physiology and disease. While plasma proteomics has advanced biomarker discovery, the analysis of cardiac tissue is essential for mechanistic understanding and therapeutic target identification; however, proteomic investigation of cardiac tissue faces unique challenges, including limited sample availability, regional heterogeneity, variability in collection and processing, and inconsistent reporting practices that hinder reproducibility and data integration. Here, we provide a practical framework for designing and conducting mass spectrometry-based proteomic studies of cardiac tissue and primary cardiac cells. We outline best practices and key considerations for sample handling, experimental design, data acquisition, quality control and statistical analysis. This guideline aims to support cardiac researchers in generating robust and reproducible proteomics datasets that advance our understanding of cardiac biology in both physiological and pathological contexts.

Indexed as

Heart DiseasesMass SpectrometryMyocardiumProteomicsAnimalsBiomarkersHumansPractice Guidelines as TopicBiomarkers

Identifiers

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.