Evidence map›Paper›PMID 42186092›Full record

ArticleMicrobiome2026

Yeast culture promotes butyrate produced fibrolytic bacteria as intracellular hydrogen sink in the rumen.

Li Wang, Fei Li, Zhiyuan Ma, Emilio M Ungerfeld, Tianxi Zhang, Zhian Zhang, Xiaozhen Liu, Qian Zhang, Xiumin Zhang

Abstract read
In one paragraph

Article in Microbiome, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Li WangState Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, 730020, China.
Fei LiState Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, 730020, China.
Zhiyuan MaState Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, 730020, China. mzy@lzu.edu.cn.
Emilio M UngerfeldCentro Regional de Investigación Carillanca, Instituto de Investigaciones Agropecuarias INIA, 4880000, Vilcún, Chile.
Tianxi ZhangState Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, 730020, China.
Zhian ZhangState Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, 730020, China.
Xiaozhen LiuInstitute of Grassland Research, Chinese Academy of Agriculture Sciences, Huhhot, 010010, China.
Qian ZhangInstitute of Grassland Research, Chinese Academy of Agriculture Sciences, Huhhot, 010010, China.
Xiumin ZhangState Key Laboratory of Forage Breeding-By-Design and Utilization, Key Laboratory for Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, 410125, China.

Funding

The National Natural Science Foundation of China 32308686
6 · The paper itself

Abstract

backgroundYeast culture (YC) supplementation is widely adopted to mitigate rumen pH depression and alleviate the inhibition of fiber degradation under starch-rich diets. Yet, the underlying microbial mechanisms, particularly how yeast culture orchestrates fibrolytic communities and affects metabolic hydrogen flow in the rumen, remain a critical knowledge gap. Accordingly, elucidating the microbial basis by which yeast culture modulates fiber degradation and hydrogen utilization under starch-rich diets is of both theoretical and practical importance.

methodsWe conducted a study with growing lambs receiving starch-rich diets that differed only in yeast culture supplementation (CON 0%, YC 1%). We evaluated their growth performance, apparent total-tract digestibilities, rumen fermentation end-products, and the rumen metagenome.

resultsThe YC treatment increased the lambs' final body mass (P = 0.02), average daily gain (P = 0.03), digestibilities of neutral detergent fiber (P < 0.001) and acid detergent fiber (P < 0.001), and rumen pH (P < 0.05), and tended to increase organic matter digestibility (P = 0.09). In addition, total VFA concentrations, particularly butyrate, were higher at 6 h post-morning feeding (P = 0.01). Fibrolytic and hydrogenotrophic taxa (e.g., Ruminococcus_E and Quinella) and CAZyme families, including GH43, GH31, GH9, and GH35, were enriched by the YC treatment, as were bacteria involved in fiber degradation and butyrate production. Furthermore, none of the top five YC treatment-enriched bacterial genomes contained any hydrogenase genes, which indicates that this butyrogenic fibrolytic consortium is significantly different from the hydrogen-producing fiber-degrading microorganisms we are familiar with.

conclusionYeast culture supplementation promoted the proliferation of a distinct butyrogenic consortium that degrades fiber while apparently disposing intracellularly metabolic hydrogen generated during fermentation, rather than releasing it as H

Indexed as

BacteriaButyratesHydrogenRumenYeastsAnimal FeedAnimalsDietary FiberDigestionFermentationHydrogen-Ion ConcentrationSheepStarchButyratesDietary FiberHydrogenStarchButyrogenesisHydrogenRumenSubacute ruminal acidosisYeast culture

Identifiers

PMID42186092
PMCPMC13383528

What Socratic holds

Textmetadata
LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.