ArticleBiosensors2026
Angle-Dependent Dip Coating Strategy for Silver Nanostructured Surface Fabrication with Enhanced Fluorescence and Surface-Enhanced Raman Scattering Properties.
Article in Biosensors, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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5 authors.
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Abstract
Noble metal nanostructures based on localized surface plasmon resonance (LSPR) can induce metal-enhanced fluorescence (MEF) and surface-enhanced Raman scattering (SERS), significantly improving trace detection sensitivity for biomedical and chemical analysis. While self-assembly of noble metal nanoparticles offers simplicity and low equipment dependence, achieving large-area, uniform, and controllable nanostructures remains challenging. In this study, angle-dependent dip coating (ADDC) technology was employed to achieve efficient, controllable self-assembly of silver nanoparticles (AgNPs) on glass slides, establishing a fabrication process for MEF/SERS dual-functional substrates. A stable AgNPs-anhydrous ethanol suspension was prepared and extracted from an inclined substrate reservoir using a microfluidic syringe pump, enabling large-area uniform nanostructure assembly. Systematic investigation revealed that substrate inclination angle provides better morphology and fluorescence enhancement control than withdrawal flow rate. The silver nanostructured surface fabricated under a withdrawal flow rate of 16 mL/h and a substrate inclination angle of 30° exhibited a Cy3 detection limit as low as 10-1 nM, with an enhancement factor ranging from 19.14 to 28.66, as well as an R6G SERS detection limit of 10-10 M with an enhancement factor of 4.07 × 108. This study confirms that ADDC technology enables simple, efficient, large-area uniform AgNPs self-assembly for superior dual-function enhancement substrates, offering a cost-effective and efficient strategy for highly sensitive trace detection.
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