Impact of DNA Extraction Strategies on Genomic and Bioinformatic Outcomes in Eight Selected Fungal Strains.

Cyrine Abid, Hela Zouari-Mechichi, Riadh Benmarzoug, Tahar Mechichi, Najla Kharrat

Abstract read

In one paragraph

Article in Journal of fungi (Basel, Switzerland), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Cyrine AbidNational School of Engineers of Sfax, University of Sfax, Soukra Road Km 4, P.O. Box 1173, Sfax 3038, Tunisia.ORCID 0000-0003-2358-6510

Hela Zouari-MechichiLaboratory of Biochemistry and Enzymatic Engineering of Lipases, National School of Engineers of Sfax, University of Sfax, Soukra Road Km 4, P.O. Box 1173, Sfax 3038, Tunisia.ORCID 0000-0002-5961-9532

Riadh BenmarzougLaboratory of Molecular and Cellular Screening Process, Centre of Biotechnology of Sfax, University of Sfax, Sidi Mansour Road Km 6, P.O. Box 1177, Sfax 3018, Tunisia.ORCID 0000-0003-2184-6323

Tahar MechichiLaboratory of Biochemistry and Enzymatic Engineering of Lipases, National School of Engineers of Sfax, University of Sfax, Soukra Road Km 4, P.O. Box 1173, Sfax 3038, Tunisia.ORCID 0000-0001-5602-4528

Najla KharratLaboratory of Molecular and Cellular Screening Process, Centre of Biotechnology of Sfax, University of Sfax, Sidi Mansour Road Km 6, P.O. Box 1177, Sfax 3018, Tunisia.ORCID 0000-0003-1570-2358

Funding

NGS-4-ECOPROD Project, From Next Generation Sequencing Microorganisms Towards Eco-friendly Biotech Based Products, which has received funding from the European's Union's Horizon Europe Research and Innovation Program 101079425

6 · The paper itself

Abstract

High-quality genomic DNA extraction remains a major bottleneck for fungal genomics, particularly for worldwide aerobic and non-photosynthetic mushroom species that rely on their rigid cell walls, interference between metabolites, polysaccharides, etc., and complex genomes. This study systematically compares five DNA extraction protocols involving four distinct sample preparation procedures (fresh (A), filtered (B), frozen (C) and cryogenic mycelium (D)) across mycelial cultures of eight Tunisian fungal strains representing

Indexed as

bioinformaticsIlluminamyceliumnanoporePCR amplification

Identifiers

PMID42187781

DOI10.3390/jof12050299

PMCPMC13208813

What Socratic holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.