ArticleSensors (Basel, Switzerland)2026
Label-Free Optical Sensor for Real-Time Monitoring of Insulin Secretion from Single Human Pancreatic Islets.
Mark F Coughlan, Lei Zhang, Umar Khan, Xuejun Zhang, Paul K Upputuri, Maria Glyavina, Yuri N Zakharov, Le Qiu, Lev T Perelman
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In one paragraphArticle in Sensors (Basel, Switzerland), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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5 · Who and what moneyAuthors and funding
9 authors.
Mark F CoughlanCenter for Advanced Biomedical Imaging and Photonics, Beth Israel Deaconess Medical Center, Harvard University, Boston, MA 02115, USA.
Lei ZhangCenter for Advanced Biomedical Imaging and Photonics, Beth Israel Deaconess Medical Center, Harvard University, Boston, MA 02115, USA.
Umar KhanCenter for Advanced Biomedical Imaging and Photonics, Beth Israel Deaconess Medical Center, Harvard University, Boston, MA 02115, USA.
Xuejun ZhangCenter for Advanced Biomedical Imaging and Photonics, Beth Israel Deaconess Medical Center, Harvard University, Boston, MA 02115, USA.ORCID 0000-0002-6268-7309 Paul K UpputuriCenter for Advanced Biomedical Imaging and Photonics, Beth Israel Deaconess Medical Center, Harvard University, Boston, MA 02115, USA.ORCID 0000-0003-3091-7023 Maria GlyavinaCenter for Advanced Biomedical Imaging and Photonics, Beth Israel Deaconess Medical Center, Harvard University, Boston, MA 02115, USA.
Yuri N ZakharovCenter for Advanced Biomedical Imaging and Photonics, Beth Israel Deaconess Medical Center, Harvard University, Boston, MA 02115, USA.
Le QiuCenter for Advanced Biomedical Imaging and Photonics, Beth Israel Deaconess Medical Center, Harvard University, Boston, MA 02115, USA.
Lev T PerelmanCenter for Advanced Biomedical Imaging and Photonics, Beth Israel Deaconess Medical Center, Harvard University, Boston, MA 02115, USA.
Funding
Endoscopic Fine-Needle Polarized Scanning Spectroscopy for Pancreatic Cystic Lesions DiagnosisR01EB025173 · NIBIB · BETH ISRAEL DEACONESS MEDICAL CENTER · PI Lev T Perelman · 2017 to 2026
$4.7MSpectro-Holographic Instrument for Dynamic Sensing of Cancer ProgressionR01CA228029 · NCI · BETH ISRAEL DEACONESS MEDICAL CENTER · PI PERELMAN, LEV T · 2018 to 2023
$3.4MMultispectral imaging of bile duct epithelial morphology for early detection of malignancyR01CA293050 · NCI · BETH ISRAEL DEACONESS MEDICAL CENTER · PI Lev T Perelman · 2024 to 2026
$2.1MA lab-on-a-chip device for measuring multiple biochemical and biophysical biomarkers of Alzheimer’s diseaseR21AG085089 · NIA · BETH ISRAEL DEACONESS MEDICAL CENTER · PI Mark Coughlan · 2024 to 2026
$668kNCI NIH HHS R01 CA228029NCI NIH HHS R01 CA293050NIA NIH HHS R21 AG085089NIBIB NIH HHS R01 EB025173NIH HHS R01 CA228029NIH HHS R01 CA293050NIH HHS R01 EB025173NIH HHS R21 AG085089US National Science Foundation CBET 2220273US National Science Foundation CBET 2325317
6 · The paper itselfAbstract
Glucose-stimulated insulin secretion is the central functional readout of pancreatic islets, yet existing assays often require offline processing or pooling of multiple islets, limiting real-time assessment of single-islet function. Here we report a microscopy-compatible islet-on-a-chip (IOC) integrated with light scattering-based broadband backscattering confocal microscopy (BBCM) for continuous, label-free optical readout of insulin secretion dynamics in functional human islets. Fabricated using two-photon polymerization, the IOC-BBCM sensor stabilizes single human islets under continuous perfusion for high-resolution optical interrogation. The sensor identifies insulin-rich β-cells label-free, as confirmed by insulin immunostaining, and monitors granule depletion and redistribution during glucose and potassium chloride (KCl) stimulation, matching ELISA-quantified insulin secretion from the same perfused islets. This modular sensor provides a non-destructive, label-free approach for monitoring stimulus-linked secretion dynamics from individual human islets and should support longitudinal studies of islet function.
Indexed as
Biosensing TechniquesInsulinIslets of LangerhansGlucoseHumansInsulin SecretionMicroscopy, ConfocalPotassium ChlorideGlucoseInsulinPotassium Chlorideinsulinislet-on-a-chiplabel-freemicroscopy
Identifiers
PMID42197878
PMCPMC13211296
What Socratic holds
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LicenceCC BY
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