Evidence map›Paper›PMID 42206182›Full record

ArticleFrontiers in pharmacology2026

Establishing a TaqMan qPCR method for identification and quantification of

Cuiping Yan, Jiabing Xu, Rong Qu, Shuchen Duan, Hui Ye, Feng Wei, Wenjuan Zhang

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Article in Frontiers in pharmacology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Cuiping YanTaizhou Institute for Drug Control, Taizhou, China.
Jiabing XuTaizhou Institute for Drug Control, Taizhou, China.
Rong QuTaizhou Institute for Drug Control, Taizhou, China.
Shuchen DuanSchool of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, China.
Hui YeTaizhou Institute for Drug Control, Taizhou, China.
Feng WeiNational Institutes for Food and Drug Control, Beijing, China.
Wenjuan ZhangNational Institutes for Food and Drug Control, Beijing, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Methods: A highly specific TaqMan real-time quantitative polymerase chain reaction (qPCR) method was established to identify HBBM from ZBM based on the 47 bp amplicon located on the internal transcribed spacer region of the nuclear ribosomal DNA. Results: The qPCR assay developed herein could specifically distinguish ZBM from HBBM. The sensitivity study showed that the detectable DNA template concentration of HBBM for this assay was 0.001 ng/μL. The efficiency of the optimized qPCR assay was evaluated to be 90.2% (R Conclusion: The TaqMan real-time qPCR assay developed in this study has high efficiency, sensitivity, and specificity; this method is applicable for not only authentication of ZBM and HBBM but also quantitative detection of a binary mixture or adulterated botanical drugs. Furthermore, it provides a reference for qualitative control of other traditional Chinese medicines.

Indexed as

adulterationFritillaria hupehensisFritillaria thunbergii Miq.identificationquantificationTaqMan real-time qPCR

Identifiers

PMID42206182
PMCPMC13201517

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.