Evidence map›Paper›PMID 42210065›Full record

ArticleBMC molecular and cell biology2026

A goat-derived gamma-tubulin antibody for triple-channel imaging of primary cilia.

Eliza Karapetian, Christoph Gerhardt, Natalia Lemanska, Monia Budnik, Erik Stahl, Jonas Füner, Stefan Hirschberg, Thorsten Pfirrmann

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC molecular and cell biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Eliza Karapetian *Health and Medical University, Potsdam, Germany.
Christoph Gerhardt *Health and Medical University, Potsdam, Germany.
Natalia LemanskaPreclinics Certified products GmbH, Potsdam, Germany.
Monia BudnikBehring Campus Eystrup GmbH, Eystrup, Germany.
Erik StahlPreclinics Gesellschaft für präklinische Forschung mbH, Potsdam, Germany.
Jonas FünerPreclinics Gesellschaft für präklinische Forschung mbH, Potsdam, Germany.
Stefan HirschbergPreclinics Certified products GmbH, Potsdam, Germany.
Thorsten PfirrmannHealth and Medical University, Potsdam, Germany. thorsten.pfirrmann@hmu-potsdam.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundPrimary cilia are essential sensory organelles, and their dysfunction leads to a broad spectrum of diseases known as ciliopathies. Immunofluorescence imaging of ciliary substructures is a key tool in cilia research, but multiplex staining is often limited by the availability of antibodies from different host species.

methodsWe developed a novel goat-derived gamma tubulin antibody for basal body labelling and validated its specificity through triple-label immunofluorescence in different cell lines.

resultsThe antibody showed strong, specific staining of basal bodies under methanol fixation, enabling clear visualization in combination with rabbit- and mouse-derived antibodies.

conclusionsThis antibody expands multiplex imaging capabilities for ciliary substructures, supporting both cilia research and diagnostics of ciliopathies.

Indexed as

AntibodiesCiliaTubulinAnimalsBasal BodiesCell LineFluorescent Antibody TechniqueGoatsHumansMiceAntibodiesTubulinAntibody generationCentrosomeCiliopathiesdSTORM microscopyGamma tubulinImmunofluorescenceMultiplex imagingPrimary cilium

Identifiers

PMID42210065
PMCPMC13217945

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.