Evidence map›Paper›PMID 42210280›Full record

ArticleJournal of nanobiotechnology2026

Single-cell and spatial transcriptomics reveal Thbs1-CD36 crosstalk between Kupffer and hepatic stellate cells following mesenchymal stem cell-derived small extracellular vesicles transplantation, alleviating liver fibrosis.

Lijun Chen, Ning Zhang, Yuqi Huang, Jingjing Qu, Yangxin Fang, Yin Yuan, Jiamin Fu, Dalong Wan, Qi Zhang, Lu Chen and 11 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of nanobiotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

21 authors.

Lijun Chen *State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, China-Singapore Belt and Road Joint Laboratory on Infection Research and Drug Development, National Medical Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310003, Zhejiang, P.R. China.
Ning Zhang *State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, China-Singapore Belt and Road Joint Laboratory on Infection Research and Drug Development, National Medical Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310003, Zhejiang, P.R. China.
Yuqi HuangState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, China-Singapore Belt and Road Joint Laboratory on Infection Research and Drug Development, National Medical Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310003, Zhejiang, P.R. China.
Jingjing QuDepartment of Respiratory Disease, Thoracic Disease Centre, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310003, Zhejiang, P.R. China.
Yangxin FangState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, China-Singapore Belt and Road Joint Laboratory on Infection Research and Drug Development, National Medical Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310003, Zhejiang, P.R. China.
Yin YuanState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, China-Singapore Belt and Road Joint Laboratory on Infection Research and Drug Development, National Medical Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310003, Zhejiang, P.R. China.
Jiamin FuState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, China-Singapore Belt and Road Joint Laboratory on Infection Research and Drug Development, National Medical Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310003, Zhejiang, P.R. China.
Dalong WanDepartment of Hepatobiliary and Pancreatic Surgery, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310003, Zhejiang, P.R. China.
Qi ZhangState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, China-Singapore Belt and Road Joint Laboratory on Infection Research and Drug Development, National Medical Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310003, Zhejiang, P.R. China.
Lu ChenInnovative Precision Medicine (IPM) Group, Hangzhou, 311215, Zhejiang, P.R. China.
Zuoshi WenDepartment of Cardiology, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310003, Zhejiang, P.R. China.
Xin ChenDepartment of Hematology, Affiliated Hangzhou First People's Hospital, School of Medicine, Westlake University, Hangzhou, 310006, Zhejiang, P.R. China.
Li YuanInnovative Precision Medicine (IPM) Group, Hangzhou, 311215, Zhejiang, P.R. China.
Zhenyu XuInnovative Precision Medicine (IPM) Group, Hangzhou, 311215, Zhejiang, P.R. China.
Yifei LiState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, China-Singapore Belt and Road Joint Laboratory on Infection Research and Drug Development, National Medical Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310003, Zhejiang, P.R. China.
Chenxia HuState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, China-Singapore Belt and Road Joint Laboratory on Infection Research and Drug Development, National Medical Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310003, Zhejiang, P.R. China.
Huadong YanDepartment of Infectious Diseases, Affiliated to Shulan International Medical College, the Shulan (Hangzhou) Hospital, Zhejiang Shuren University, Hangzhou, 310022, Zhejiang, P.R. China.
Hiromi IzawaDepartment of Immunoregulation, Institute of Medical Science, Tokyo Medical University, Shinjuku-ku, Tokyo, 160-8402, Japan.
Takayuki YoshimotoDepartment of Immunoregulation, Institute of Medical Science, Tokyo Medical University, Shinjuku-ku, Tokyo, 160-8402, Japan.
Lanjuan LiState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, China-Singapore Belt and Road Joint Laboratory on Infection Research and Drug Development, National Medical Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310003, Zhejiang, P.R. China. ljli@zju.edu.cn.
Charlie XiangState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, China-Singapore Belt and Road Joint Laboratory on Infection Research and Drug Development, National Medical Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310003, Zhejiang, P.R. China. cxiang@zju.edu.cn.

Funding

Fundamental Research Funds for the Central Universities 2022ZFJH003Fundamental Research Funds for the Central Universities 2025ZFJH03National Key Research and Development Program of China 2022YFA1105603Natural Science Foundation of Zhejiang Province LTGY24H030002
6 · The paper itself

Abstract

backgroundSmall extracellular vesicles (sEVs) derived from mesenchymal stem cells represent a novel regenerative therapeutic strategy for various liver injuries. Menstrual blood-derived mesenchymal stem cells (MenSCs)-derived EVs may have therapeutic potential in treating liver fibrosis. In this study, systemically injected MenSCs-derived sEVs migrated into the injured liver, ameliorated serological indices, and decreased collagenous fiber accumulation in carbon tetrachloride (CCl

resultsSingle-cell RNA sequencing (scRNA-seq) and spatial transcriptomics were combined to investigate the mechanism by which classical fibrosis-related genes are reduced to inhibit liver fibrosis, targeting Kupffer cells and hepatic stellate cells (HSCs). Fourteen major cellular clusters were determined in the livers of the control (or con), CCl

conclusionsIn this study, we identified interacting target cells (Kupffer cells and HSCs), signaling molecules (Thbs1-CD36), and underlying therapeutic targets of MenSC-sEVs in treating liver fibrosis.

Indexed as

CD36 AntigensExtracellular VesiclesHepatic Stellate CellsKupffer CellsLiver CirrhosisMesenchymal Stem CellsThrombospondin 1AnimalsCarbon TetrachlorideFemaleHumansMaleMiceMice, Inbred C57BLSingle-Cell AnalysisSpatial TranscriptomicsCarbon TetrachlorideCD36 AntigensThrombospondin 1thrombospondin-1, humanExtracellular vesicles-based therapyHepatic stellate cell (HSC)Kupffer cellLiver fibrosisMenstrual blood-derived MSCsSingle-cell RNA sequencingSmall extracellular vesiclesSpatial transcriptomicsThbs1/CD36 crosstalk

Identifiers

PMID42210280
PMCPMC13430791

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.