Evidence map›Paper›PMID 42210370›Full record

ArticleJournal of animal science and biotechnology2026

Integrative analysis of RNA-seq and Ribo-seq reveals that lncRNA regulates chicken myogenesis through encoding peptide.

Yufang Niu, Liyang He, Zhiyuan An, Meng Yuan, Haigang Ji, Wei Wang, Chengjie Wei, Ruili Han, Weihua Tian, Yadong Tian and 3 more

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PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of animal science and biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Yufang Niu *College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450046, China.
Liyang He *College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450046, China.
Zhiyuan AnCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450046, China.
Meng YuanCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450046, China.
Haigang JiCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450046, China.
Wei WangCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450046, China.
Chengjie WeiCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450046, China.
Ruili HanCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450046, China.
Weihua TianCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450046, China.
Yadong TianCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450046, China.
Xiaojun LiuCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450046, China.
Xiangtao KangCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450046, China.
Zhuanjian LiCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450046, China. lizhuanjian@163.com.

Funding

National Key Research and Development Program of China 2024YFF1000100National Natural Science Foundation of China 32441084Science and Technology Innovation Leading Talent Support Program of Henan Province Grant No. 254000510050
6 · The paper itself

Abstract

backgroundLong noncoding RNAs (lncRNAs) participate in various critical regulatory steps during myogenesis. LncRNAs can encode small peptides, which can regulate gene expression through multiple mechanisms, thereby participating in key biological processes.

resultsThe lncRNAs were screened between proliferating and differentiating myoblast in chicken through RNA-seq and Ribo-seq. As a result, 178 DE-lncRNAs were identified in RNA-seq, and three of them were identified as differentially translated lncRNAs by Ribo-seq. Among them, lncMPD, which showed coding potential, was highly expressed in proliferating myoblast. It encoded a small peptide containing 74 amino acids, which was named MPD-74aa. MPD-74aa was validated via WB and mass spectrometry. We subsequently confirmed that MPD-74aa promotes myoblast proliferation and inhibits its differentiation. Co-IP revealed that MPD-74aa interacts with the protein CDK1. Moreover, MPD-74aa positively regulated the expression of CDK1.

conclusionThis study confirms that the lncMPD plays a crucial regulatory role in the chicken myogenesis by encoding the small peptide MPD-74aa. Mechanistically, MPD-74aa exerts its regulatory function through interaction with CDK1, a key protein marker of cell proliferation. These findings provide new insight into the molecular mechanisms about the coding capacity of lncRNA regulating chicken muscle development.

Indexed as

DifferentiationLncRNAMPD-74aaMyoblastsProliferation

Identifiers

PMID42210370
PMCPMC13220464

What Socratic holds

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