ArticleFrontiers in physiology2025
Intestinal gene expression in heat-stressed broilers selected for high water efficiency.
Article in Frontiers in physiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Sometimes it's good to be lucky: blood flow, glutathione, oxidative stress, and mitochondria.Frontiers in physiology · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background: The major goal of this study was to compare mRNA expression associated with water homeostasis in intestines of high water efficient (HWE) broilers to expression in unselected Modern Random Bred (MRB) broilers under thermoneutral (TN) and heat stress (HS) environments. Methods: A 2 × 2 factorial study was conducted with two lines (MRB and HWE) and environments (TN and HS). Males were raised in environmental chambers and maintained in TN conditions from 0 to 4 weeks. From 4 to 7 weeks, broilers were maintained under TN conditions (25C) or exposed to cyclic HS conditions (35C, 8 h/d). At 7 weeks, upper and lower duodenum, and cecum samples were obtained. mRNA was determined by RT-PCR for; 1) heat shock proteins (HSP70, HSP90), 2) the arginine vasotocin (AVT), mesotocin (MT) and receptors (VR2, VR1a, VR1b, MTR), 3) the renin angiotensin system (RAS): angiotensinogen (AGT), angiotensin receptors, (AT1, AT2), renin (REN), AGT converting enzyme (ACE), and Na Results: A main effect of HS was observed for HSP70 and HSP90 expression in all three intestinal-segments. In lower duodenum, HS-induced upregulation of AVT was accompanied by elevations in VR2, VR1a, VRb, AT1, AQPs (1, 3, 11) OCLN, and CLND2, CLDN15. Main effects of broiler line were observed with HWE exhibiting increased mRNA expression of VR2, VR1a, VR1b, AQP1, AQP3 and AQP5 and CLDN2 in the upper duodenum. There was also higher VR2, MT and AQP5 and lower expression of CLDN15 in the lower duodenum but there were no differences in gene expression between MRB and HWE lines in the cecum. Heat stress upregulated AVT, VR1a, MTR, AVT/MT ratio, AQP1, and AQP3 but downregulated ACE in the upper duodenum. Heat stress also upregulated AVT, VR2, VR1a, VR1b, MTR, AT1, AT2 AQP3, ACP5, OCLN, CLDN2, CLDN15 in the lower duodenum and cecum. Conclusion: The results provide insight into mechanisms responsible for water efficiency in HWE broilers and provide new information in gene expression in the gastrointestinal tract of broilers exposed to chronic cyclic heat stress conditions.
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.