Evidence map›Paper›PMID 42213656›Full record

ArticlePLOS global public health2026

Target product profiles of laboratory and data analytical frameworks for genotyping to monitor antimalarial efficacy.

Mateusz M Plucinski, Amy Wesolowski, Inna Gerlovina, Aimee R Taylor, Jessica Briggs, Andrés Aranda-Díaz, Monica Golumbeanu, Marko Bajic, Jeffrey A Bailey, Joel L N Barratt and 19 more

Abstract read
In one paragraph

Article in PLOS global public health, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

29 authors.

Mateusz M PlucinskiMalaria Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America.
Amy WesolowskiDepartment of Epidemiology, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, United States of America.ORCID https://orcid.org/0000-0001-6320-3575
Inna GerlovinaDivision of HIV, ID, and Global Medicine, Department of Medicine, University of California San Francisco, San Francisco, California, United States of America.
Aimee R TaylorDepartment of Global Health, Institut Pasteur, Université Paris Cité, Paris, France.
Jessica BriggsDivision of HIV, ID, and Global Medicine, Department of Medicine, University of California San Francisco, San Francisco, California, United States of America.
Andrés Aranda-DíazISGlobal, Barcelona, Spain.
Monica GolumbeanuDepartment of Epidemiology and Public Health, Swiss Tropical and Public Health Institute, Allschwil, Switzerland.ORCID https://orcid.org/0000-0002-8459-9983
Marko BajicMalaria Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America.ORCID https://orcid.org/0000-0002-8566-5153
Jeffrey A BaileyDepartment of Pathology and Laboratory Medicine, Brown University, Providence, Rhode Island, United States of America.
Joel L N BarrattDepartment of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia, United States of America.
Caroline BuckeeDepartment of Immunology and Infectious Disease, Harvard TH Chan School of Public Health, Boston, Massachusetts, United States of America.
Awa B DemeCentre International de Recherche et de Formation en Genomique Appliquee et de Surveillance Sanitaire, Dakar, Senegal.
Ingrid FelgerDepartment of Medical Parasitology and Infection Biology, Swiss Tropical and Public Health Institute, Allschwil, Switzerland.ORCID https://orcid.org/0000-0003-1255-2606
Anita GhansahDepartment of Parasitology, Noguchi Memorial Institute for Medical Research, College of Health Sciences, University of Ghana, Legon, Ghana.
Ian HastingsDepartment of Tropical Disease Biology, Liverpool School of Tropical Medicine, Liverpool, United Kingdom.ORCID https://orcid.org/0000-0002-1332-742X
Johanna Helena KattenbergDepartment of Biomedical Sciences, Institute of Tropical Medicine, Antwerp, Belgium.ORCID https://orcid.org/0000-0002-2971-5136
Alfredo MayorISGlobal, Barcelona, Spain.
Didier MenardMalaria Genetics and Resistance Team (MEGATEAM), UR 3073 - Pathogens Host Arthropods Vectors Interactions Unit, Université de Strasbourg, Strasbourg, France.ORCID https://orcid.org/0000-0003-1357-4495
Leah F MoriartyMalaria Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America.ORCID https://orcid.org/0000-0002-7836-1119
Daniel NeafseyDepartment of Immunology and Infectious Disease, Harvard TH Chan School of Public Health, Boston, Massachusetts, United States of America.ORCID https://orcid.org/0000-0002-1665-9323
Lucy OkellMRC Centre for Global Infectious Disease Analysis, Imperial College London, London, United Kingdom.
Isabella OyierBiosciences Department, KEMRI-Wellcome Trust Research Programme, Kilifi, Kenya.
Jaishree RamanCentre for Emerging Zoonotic and Parasitic Diseases, National Institute for Communicable Diseases, Johannesburg, South Africa.
Philip J RosenthalDivision of HIV, ID, and Global Medicine, Department of Medicine, University of California San Francisco, San Francisco, California, United States of America.
Anna Rosanas-UrgellDepartment of Biomedical Sciences, Institute of Tropical Medicine, Antwerp, Belgium.ORCID https://orcid.org/0000-0002-0432-5203
Robert VerityMRC Centre for Global Infectious Disease Analysis, Imperial College London, London, United Kingdom.
Sarah K VolkmanDepartment of Immunology and Infectious Disease, Harvard TH Chan School of Public Health, Boston, Massachusetts, United States of America.
Christian NsanzabanaDepartment of Epidemiology and Public Health, Swiss Tropical and Public Health Institute, Allschwil, Switzerland.
Bryan GreenhouseDivision of HIV, ID, and Global Medicine, Department of Medicine, University of California San Francisco, San Francisco, California, United States of America.ORCID https://orcid.org/0000-0003-0287-9111

Funding

Mentoring translational scientists in international infectious disease researchK24AI144048 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI Bryan R Greenhouse · 2019 to 2026
$1.6M
Data and analysis ecosystem for eukaryotic pathogen targeted sequencingU01AI184646 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI GREENHOUSE, BRYAN R · 2024 to 2025
$1.5M
Bill & Melinda Gates Foundation INV-009416Bill & Melinda Gates Foundation INV-037564NIAID NIH HHS K24 AI144048NIAID NIH HHS U01 AI184646
6 · The paper itself

Abstract

Therapeutic efficacy studies (TESs) are the standard to evaluate antimalarial drug efficacy and guide malaria treatment policy. TESs are particularly relevant now, with resistance to first-line regimens emerging in sub-Saharan Africa. For TESs, a range of parasite genotyping and data analyses are available for genotype correction, a process to distinguish whether recurrent parasitemia after therapy is due to recrudescence of initially infecting parasites (treatment failure) or a new infection. The choice of methods for laboratory genotyping and data analyses can have a large effect on how outcomes are classified, and thereby on trial results. The currently recommended and most widely used laboratory and analytical methods for TES genotyping do not incorporate recent methodological advances and can produce biased results. As such current TES results can be difficult to interpret, especially in areas with high malaria transmission, such as much of sub-Saharan Africa. Thus, improving the accuracy and reliability of TES genotyping and data analysis are a major priority. To that end, we present target product profiles that outline key specifications for genetic data generation, processing, and data analysis, with the goal of creating rigorous and consistent community standards. Primary recommended specifications for laboratory methods include high sensitivity, specificity, and reproducibility, and guidance on the number and genetic diversity of targets; criteria which are best and likely only met by amplicon sequencing. Primary recommendations for data analysis methods include high classification accuracy, accounting for errors in genotyping, and accounting for alleles matching by chance. All laboratory and data analysis methods used should be systematically validated and publicly documented so that TES results, which have major policy implications, can be relied upon for sound programmatic decision making.

Identifiers

PMID42213656
PMCPMC13221082

What Socratic holds

Textmetadata
LicenceCC0
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.