Evidence map›Paper›PMID 42214671›Full record

ArticleThe Journal of biological chemistry2026

Hexosamine biosynthesis drives hemocyanin O-GlcNAcylation to potentiate antibacterial immunity in shrimp.

Qian Feng, Maoshuai Fu, Wei Wang, Tingchu Wu, Junjie Nie, Zhihong Zheng, Yongzhen Zhao, Jude Juventus Aweya, Yueling Zhang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of biological chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Qian FengInstitute of Marine Sciences and Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou, China; Department of Medical Laboratory and Department of Reproductive Medicine, Luohu Clinical College of Shantou University Medical College, Shantou University, Shantou, China.
Maoshuai FuInstitute of Marine Sciences and Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou, China.
Wei WangInstitute of Marine Sciences and Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou, China.
Tingchu WuInstitute of Marine Sciences and Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou, China.
Junjie NieInstitute of Marine Sciences and Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou, China; Department of Medical Laboratory and Department of Reproductive Medicine, Luohu Clinical College of Shantou University Medical College, Shantou University, Shantou, China.
Zhihong ZhengInstitute of Marine Sciences and Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou, China.
Yongzhen ZhaoGuangxi Academy of Fishery Sciences, Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Nanning, China.
Jude Juventus AweyaInstitute of Marine Sciences and Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou, China; College of Ocean Food and Biological Engineering, Jimei University, Xiamen, Fujian, China; Department of Food and Human Nutritional Sciences, University of Manitoba, Winnipeg, Manitoba, Canada; The Canadian Centre for Agri-Food Research in Health and Medicine, St Boniface Hospital Albrechtsen Research Centre, Winnipeg, Manitoba, Canada. Electronic address: jude.aweya@umanitoba.ca.
Yueling ZhangInstitute of Marine Sciences and Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou, China. Electronic address: zhangyl@stu.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Post-translational modifications (PTMs) are key regulators of immune responses; however, their roles in invertebrate immunity remain poorly defined. Here, we show that Penaeus vannamei employs O-GlcNAcylation, a dynamic PTM controlled by the hexosamine biosynthetic pathway (HBP), to enhance antibacterial defense. Bacterial infection induces metabolic reprogramming in hemocytes, upregulating HBP enzymes, and promoting O-GlcNAcylation of hemocyanin (PvHMC) through O-GlcNAc transferase (PvOGT). Site-specific modification of the PvHMC large subunit at Thr584 enhances its conformational stability and interaction with bacterial pathogen-associated molecular patterns, including lipopolysaccharide and peptidoglycan, thereby increasing bacterial binding, agglutination, and killing. Disruption of HBP flux or OGT activity reduces hemocyanin O-GlcNAcylation and impairs bacterial clearance, whereas inhibition of O-GlcNAcase enhances O-GlcNAcylation and antibacterial efficacy. Together, these findings identify HBP-driven O-GlcNAcylation as a metabolic-immune regulatory axis in shrimp and establish hemocyanin O-GlcNAcylation as a key mechanism underlying effective innate antibacterial defense, with potential implications for disease control in aquaculture.

Indexed as

Anti-Bacterial AgentsHemocyaninsHexosaminesPenaeidaeAnimalsProtein Processing, Post-TranslationalAnti-Bacterial AgentsHemocyaninsHexosaminesantibacterial immunityhemocyaninhexosamine biosynthetic pathwayhost-pathogen interactionmetabolic reprogrammingO-GlcNAcylationPenaeus vannameipost-translational modification

Identifiers

PMID42214671
PMCPMC13310628

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.